Cloned (Comment) | Organism |
---|---|
the nitrite transporting system is encoded by the four genes nrtA, -B, -C, and -D, organized in the CynABD operon genes, physical map of the cynABDS genomic region | Synechococcus elongatus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the NA3 mutant of strain PCC7942, constructed by deleting the nrtABCD genes from the nirA operon, is defective in the active transport of nitrate, an insertional mutant of NA3 is constructed for each of the four genes and analyzed for its nitrite transport ability, overview | Synechococcus elongatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cyanate | competitively inhibits nitrite transport | Synechococcus elongatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
nitrite | about | Synechococcus elongatus | |
0.001 | - |
nitrate | about | Synechococcus elongatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + nitrate/out | Synechococcus elongatus | the enzyme transports both nitrate and nitrite with high affinity | ADP + phosphate + nitrate/in | - |
? | |
ATP + H2O + nitrate/out | Synechococcus elongatus PCC 7942 | the enzyme transports both nitrate and nitrite with high affinity | ADP + phosphate + nitrate/in | - |
? | |
ATP + H2O + nitrite/out | Synechococcus elongatus | the enzyme transports both nitrate and nitrite with high affinity | ADP + phosphate + nitrite/in | - |
? | |
ATP + H2O + nitrite/out | Synechococcus elongatus PCC 7942 | the enzyme transports both nitrate and nitrite with high affinity | ADP + phosphate + nitrite/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus elongatus | - |
strain PCC7942, ABC-type bispecific nitrate/nitrite transporter is encoded by the four genes nrtA, -B, -C, and -D | - |
Synechococcus elongatus PCC 7942 | - |
strain PCC7942, ABC-type bispecific nitrate/nitrite transporter is encoded by the four genes nrtA, -B, -C, and -D | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + nitrate/out | the enzyme transports both nitrate and nitrite with high affinity | Synechococcus elongatus | ADP + phosphate + nitrate/in | - |
? | |
ATP + H2O + nitrate/out | the enzyme transports both nitrate and nitrite with high affinity | Synechococcus elongatus PCC 7942 | ADP + phosphate + nitrate/in | - |
? | |
ATP + H2O + nitrite/out | the enzyme transports both nitrate and nitrite with high affinity | Synechococcus elongatus | ADP + phosphate + nitrite/in | - |
? | |
ATP + H2O + nitrite/out | the enzyme transports both nitrate and nitrite with high affinity | Synechococcus elongatus PCC 7942 | ADP + phosphate + nitrite/in | - |
? | |
ATP + H2O + NO2-/out | - |
Synechococcus elongatus | ADP + phosphate + NO2-/in | - |
? | |
ATP + H2O + NO2-/out | - |
Synechococcus elongatus PCC 7942 | ADP + phosphate + NO2-/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABC-type nitrate/nitrite-bispecific transporter | - |
Synechococcus elongatus |
ABC-type NRT | - |
Synechococcus elongatus |
ATP-binding cassette-type nitrate/nitrite-bispecific transporter | - |
Synechococcus elongatus |
CynABD transporter | - |
Synechococcus elongatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Synechococcus elongatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 9.6 | assay at, medium pH | Synechococcus elongatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Synechococcus elongatus |