Literature summary for 7.2.4.5 extracted from

Bendrat, K.; Buckel, W.
Cloning, sequencing and expression of the gene encoding the carboxytransferase subunit of the biotin-dependent Na+ pump glutaconyl-CoA decarboxylase from Acidaminococcus fermentans in Escherichia coli (1993), Eur. J. Biochem., 211, 697-702 .

Search for more literature for 7.2.4.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Acidaminococcus fermentans

Inhibitors

Inhibitors	Comment	Organism	Structure
N-ethylmaleimide	Gcd holoenzyme loses half of its activity within 6 min, whereas the expressed GcdA requires 21 min	Acidaminococcus fermentans

Organism

Organism	UniProt	Comment	Textmining
Acidaminococcus fermentans	Q06700 and Q9ZAA6 and Q9ZAA7 and Q9ZAA8	Q06700 i.e. subunit GcdA, Q9ZAA6 .e. subunit GcdB, Q9ZAA7 .e. subunit GcdC, Q9ZAA8 .e. subunit GcdD	-
Acidaminococcus fermentans DSM 20731	Q06700 and Q9ZAA6 and Q9ZAA7 and Q9ZAA8	Q06700 i.e. subunit GcdA, Q9ZAA6 .e. subunit GcdB, Q9ZAA7 .e. subunit GcdC, Q9ZAA8 .e. subunit GcdD	-

Subunits

Subunits	Comment	Organism
?	enzyme is composed of subunits GcdA (65 kDa), GcdB (36 kDa), GcdC (24 kDa) and GcdD (14 kDa), calculated from sequence	Acidaminococcus fermentans

Cofactor

Cofactor	Comment	Organism	Structure
Biocytin	may substitute for biotin, Km value 13 mM	Acidaminococcus fermentans
biotin	Km value 51 mM	Acidaminococcus fermentans

General Information

General Information	Comment	Organism
physiological function	sodium-ion pump glutaconyl-CoA decarboxylase is composed of four subunits: GcdA, the carboxytransferase (65 kDa), GcdB, the carboxylyase (36 kDa), GcdC, the biotin carrier (24 kDa) and GcdD (14 kDa) of unknown function. The GcdA subunit catalyzes the biotin-dependent formation of crotonyl-CoA from glutaconyl-CoA	Acidaminococcus fermentans

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Release 2023.1 (January 2023)