Activating Compound | Comment | Organism | Structure |
---|---|---|---|
biotin | biotinyl enzyme | Peptoniphilus asaccharolyticus | |
biotin | biotinyl enzyme | Fusobacterium nucleatum | |
biotin | biotinyl enzyme | [Clostridium] symbiosum | |
biotin | biotinyl enzyme | Acidaminococcus fermentans | |
Brij 35 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Peptoniphilus asaccharolyticus | |
Brij 35 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Fusobacterium nucleatum | |
Brij 35 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | [Clostridium] symbiosum | |
Brij 35 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Acidaminococcus fermentans | |
Triton X-100 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Peptoniphilus asaccharolyticus | |
Triton X-100 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Fusobacterium nucleatum | |
Triton X-100 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | [Clostridium] symbiosum | |
Triton X-100 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Acidaminococcus fermentans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Avidin | not: avidin biotin complex | Acidaminococcus fermentans | |
Avidin | not: avidin biotin complex | [Clostridium] symbiosum | |
Avidin | not: avidin biotin complex | Fusobacterium nucleatum | |
Avidin | not: avidin biotin complex | Peptoniphilus asaccharolyticus | |
deoxycholate | at 20-30°C | Acidaminococcus fermentans | |
deoxycholate | at 20-30°C | [Clostridium] symbiosum | |
deoxycholate | at 20-30°C | Peptoniphilus asaccharolyticus | |
ethanol | - |
Acidaminococcus fermentans | |
ethanol | - |
[Clostridium] symbiosum | |
ethanol | - |
Fusobacterium nucleatum | |
ethanol | - |
Peptoniphilus asaccharolyticus | |
N-Alkanols | concentration required for complete inactivation decreases N-dodecyl-N-dimethylaminoxide at 20-30°C | Acidaminococcus fermentans | |
N-ethylmaleimide | - |
Acidaminococcus fermentans | |
N-ethylmaleimide | - |
[Clostridium] symbiosum | |
N-ethylmaleimide | - |
Fusobacterium nucleatum | |
N-ethylmaleimide | - |
Peptoniphilus asaccharolyticus | |
SDS | at 20-30°C | Acidaminococcus fermentans | |
SDS | at 20-30°C | [Clostridium] symbiosum | |
SDS | at 20-30°C | Peptoniphilus asaccharolyticus | |
Triton X-114 | at 20-30°C | Acidaminococcus fermentans | |
Triton X-114 | at 20-30°C | [Clostridium] symbiosum | |
Triton X-114 | at 20-30°C | Peptoniphilus asaccharolyticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Peptoniphilus asaccharolyticus | 16020 | - |
membrane | - |
Fusobacterium nucleatum | 16020 | - |
membrane | - |
[Clostridium] symbiosum | 16020 | - |
membrane | - |
Acidaminococcus fermentans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Li+ | activates, Km: 100 mM | Peptoniphilus asaccharolyticus | |
Li+ | activates, Km: 100 mM | Fusobacterium nucleatum | |
Li+ | activates, Km: 100 mM | [Clostridium] symbiosum | |
Li+ | activates, Km: 100 mM | Acidaminococcus fermentans | |
Na+ | Km: 1 mM | Acidaminococcus fermentans | |
Na+ | stimulates | Peptoniphilus asaccharolyticus | |
Na+ | stimulates | Fusobacterium nucleatum | |
Na+ | stimulates | [Clostridium] symbiosum | |
Na+ | stimulates | Acidaminococcus fermentans | |
Na+ | activation by Na+ is inhibited by Li+ | Peptoniphilus asaccharolyticus | |
Na+ | activation by Na+ is inhibited by Li+ | Fusobacterium nucleatum | |
Na+ | activation by Na+ is inhibited by Li+ | [Clostridium] symbiosum | |
Na+ | activation by Na+ is inhibited by Li+ | Acidaminococcus fermentans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
in sucrose density gradient centrifugation the activity is recovered in 2 peaks: MW 470000, possibly dimer and MW 250000, possibly monomer | Acidaminococcus fermentans |
35000 | - |
1 * 120000-140000, alpha, contains biotin + 1 * 60000, beta, + 1 * 35000, gamma, hydrophobic, SDS-PAGE | Acidaminococcus fermentans |
60000 | - |
1 * 120000-140000, alpha, contains biotin + 1 * 60000, beta, + 1 * 35000, gamma, hydrophobic, SDS-PAGE | Acidaminococcus fermentans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaconyl-CoA + Na+[side 1] | Peptoniphilus asaccharolyticus | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
glutaconyl-CoA + Na+[side 1] | Fusobacterium nucleatum | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
glutaconyl-CoA + Na+[side 1] | [Clostridium] symbiosum | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
glutaconyl-CoA + Na+[side 1] | Acidaminococcus fermentans | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidaminococcus fermentans | - |
ATCC 25085 | - |
Fusobacterium nucleatum | - |
- |
- |
Peptoniphilus asaccharolyticus | - |
- |
- |
[Clostridium] symbiosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acidaminococcus fermentans |
Storage Stability | Organism |
---|---|
-20°C, little loss of activity after 1 year | Acidaminococcus fermentans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
Peptoniphilus asaccharolyticus | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
Fusobacterium nucleatum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
[Clostridium] symbiosum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
Acidaminococcus fermentans | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
glutaconyl-CoA + Na+[side 1] | - |
Peptoniphilus asaccharolyticus | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
glutaconyl-CoA + Na+[side 1] | - |
Fusobacterium nucleatum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
glutaconyl-CoA + Na+[side 1] | - |
[Clostridium] symbiosum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
glutaconyl-CoA + Na+[side 1] | - |
Acidaminococcus fermentans | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
additional information | not: 3-methylglutaconyl-CoA | Peptoniphilus asaccharolyticus | ? | - |
? | |
additional information | not: 3-methylglutaconyl-CoA | Fusobacterium nucleatum | ? | - |
? | |
additional information | not: 3-methylglutaconyl-CoA | [Clostridium] symbiosum | ? | - |
? | |
additional information | not: 3-methylglutaconyl-CoA | Acidaminococcus fermentans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Fusobacterium nucleatum |
? | 1 * 120000-140000, alpha, contains biotin + 1 * 60000, beta, + 1 * 35000, gamma, hydrophobic, SDS-PAGE | Acidaminococcus fermentans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Peptoniphilus asaccharolyticus |
25 | - |
assay at | Fusobacterium nucleatum |
25 | - |
assay at | [Clostridium] symbiosum |
25 | - |
assay at | Acidaminococcus fermentans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Peptoniphilus asaccharolyticus |
7 | - |
assay at | Fusobacterium nucleatum |
7 | - |
assay at | [Clostridium] symbiosum |
7 | - |
assay at | Acidaminococcus fermentans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | biotinyl enzyme | Peptoniphilus asaccharolyticus | |
biotin | biotinyl enzyme | Fusobacterium nucleatum | |
biotin | biotinyl enzyme | [Clostridium] symbiosum | |
biotin | biotinyl enzyme | Acidaminococcus fermentans |