Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M672G/M674A/H676A/H677G/H682A/H683G | the mutant shows decreased affinity for copper. The mutations within the His/Met-rich segment Met672-Pro707 (HM-loop) do not prevent the ability of the enzyme to form a phosphorylated intermediate during ATP hydrolysis but inhibit subsequent dephosphorylation, a step associated with copper release | Homo sapiens |
M687A/M692A | the mutant shows decreased affinity for copper. The mutations within the His/Met-rich segment Met672-Pro707 (HM-loop) do not prevent the ability of the enzyme to form a phosphorylated intermediate during ATP hydrolysis but inhibit subsequent dephosphorylation, a step associated with copper release | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | the enzyme contains two Cu(I)-binding sites. Copper binding within the His-Met-loop stabilizes Cu(I) and protects it from oxidation, which may further aid the transfer of copper from ATP7A to acceptor proteins | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q04656 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
chitin resin column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu2+/in | - |
Homo sapiens | ADP + phosphate + Cu2+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP7A | - |
Homo sapiens |
copper-transporting ATPase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of ATP7A results in the neurodegenerative disorder Menkes disease | Homo sapiens |