Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant myc-tagged ATP7Bs in COS1 cells using an adenoviral vector | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C983A/C985A | site-directed mutagenesis of CXC copper binding motif in the transmembrane copper site TM6, absence of alkali labile phosphoenzyme formation | Homo sapiens |
D1027N | site-directed mutagenesis of the P domain, absence of alkali labile phosphoenzyme formation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bathocuproine disulfonate | a copper chelator, reduced ATP7B activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu2+/in | Homo sapiens | - |
ADP + phosphate + Cu2+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | ATP7B phosphorylation with ATP occurs with diphasic kinetics and is totally copper-dependent | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant myc-tagged ATP7B is isolated from the microsomal fraction of COS-1 cell homogenates | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.081 | - |
partially purifed recombinant ATP7B in microsomes, copper-dependent, steady-state ATPase, 37°C, pH 6.0, in presence of copper and copper chelator | Homo sapiens |
0.112 | - |
partially purifed recombinant ATP7B in microsomes, copper-dependent, steady-state ATPase, 37°C, pH 6.0 in presence of copper | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu2+/in | - |
Homo sapiens | ADP + phosphate + Cu2+/out | - |
? | |
ATP + H2O + Cu2+/in | ATP7B phosphorylation with ATP occurs with diphasic kinetics and is totally copper-dependent | Homo sapiens | ADP + phosphate + Cu2+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP7B | - |
Homo sapiens |
Cu+-ATPase | - |
Homo sapiens |
Wilson disease protein | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | ATP7B is a P-type ATPase required for copper homeostasis and related to Wilson disease of humans | Homo sapiens |