Cloned (Comment) | Organism |
---|---|
Recombinant adenovirus vector, rAdATP7Bmyc, containing CMV promoter driven WT human ATP7B cDNA, fused with 30 cmyc tag, expression in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C575A/C578A | mutation in the 6th copper site of the NMBD, catalytically inactive, no phosphoenzyme intermediate formed upon addition of ATP | Homo sapiens |
C983A/C985A | mutation in the transmembrane copper binding site, TMBS, catalytically inactive, no phosphoenzyme intermediate formed upon addition of ATP | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | detection of a positive charge displacement within a single catalytic cycle of ATP7B upon addition of ATP and formation of phosphoenzyme intermediate, C983A/C985A and C575A/C578A mutants demonstrate that ATP7B activation requires a copper binding site in the N-terminus extension | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP7B | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | ATP7B is a copper dependent P-type ATPase, required for copper homeostasis | Homo sapiens |