Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Na+ | - |
Leishmania amazonensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Furosemide | - |
Leishmania amazonensis | |
sodium orthovanadate | - |
Leishmania amazonensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Leishmania amazonensis | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania amazonensis | - |
MHOM/BR/75/Josefa strain | - |
Leishmania amazonensis MHOM/BR/75/Josefa | - |
MHOM/BR/75/Josefa strain | - |
Purification (Comment) | Organism |
---|---|
plasma membranes are isolated from the promastigotes of Leishmania amazonensis | Leishmania amazonensis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00325 | - |
ATPase activity in homogenate, hydrolysis of ATP, without ouabain, control | Leishmania amazonensis |
0.00336 | - |
ATPase activity in homogenate, hydrolysis of ATP, with 1 mM ouabain, control | Leishmania amazonensis |
0.00734 | - |
ATPase activity in homogenate, hydrolysis of ATP, with 1 mM ouabain, control, 10 mM MgCl2 | Leishmania amazonensis |
0.00741 | - |
ATPase activity in homogenate, hydrolysis of ATP, without ouabain, control, 10 mM MgCl2 | Leishmania amazonensis |
0.00808 | - |
ATPase activity in homogenate, hydrolysis of ATP, with 1 mM ouabain, control, 10 mM MgCl2, 120 mM NaCl | Leishmania amazonensis |
0.00813 | - |
ATPase activity in homogenate, hydrolysis of ATP, without ouabain, control, 10 mM MgCl2, 120 mM NaCl | Leishmania amazonensis |
0.00818 | - |
ATPase activity in homogenate, hydrolysis of ATP, with 1 mM ouabain, control, 10 mM MgCl2, 120 mM NaCl, 30 mM KCl | Leishmania amazonensis |
0.00907 | - |
ATPase activity in homogenate, hydrolysis of ATP, without ouabain, control, 10 mM MgCl2, 120 mM NaCl, 30 mM KCl | Leishmania amazonensis |
0.0103 | - |
ATPase activity in plasma membranes in the presence of 1 mM ouabain, hydrolysis of ATP, control | Leishmania amazonensis |
0.0272 | - |
ATPase activity in plasma membranes in the presence of 1 mM ouabain, hydrolysis of ATP, 10 mM MgCl2 | Leishmania amazonensis |
0.0286 | - |
ATPase activity in plasma membranes in the presence of 1 mM ouabain, hydrolysis of ATP, 10 mM MgCl2, 120 mM NaCl, 30 mM KCl | Leishmania amazonensis |
0.0294 | - |
ATPase activity in plasma membranes in the presence of 1 mM ouabain, hydrolysis of ATP, 10 mM MgCl2, 120 mM NaCl | Leishmania amazonensis |
Synonyms | Comment | Organism |
---|---|---|
Na+-ATPase | - |
Leishmania amazonensis |
Na+-exporting ATPase | - |
Leishmania amazonensis |
P-type Na+-ATPase | - |
Leishmania amazonensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
ATPase activity assay | Leishmania amazonensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
ATPase activity assay | Leishmania amazonensis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0001 | - |
- |
Leishmania amazonensis | sodium orthovanadate | |
0.27 | - |
- |
Leishmania amazonensis | Furosemide |