Cloned (Comment) | Organism |
---|---|
expressed in HEK-293S GnT1- cells | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
E343A | the mutant has no activity | Sus scrofa |
E343L | the mutant has no activity | Sus scrofa |
E343Q | the mutation demonstrates reduced activity | Sus scrofa |
E343V | the mutant has no activity | Sus scrofa |
E795Q | the mutant shows increased affinity for K+ compared to the wild type enzyme | Sus scrofa |
E820D | the mutant remains active | Sus scrofa |
E820Q | the mutant has K+-independent constitutive dephosphorylation activity and an increased preference for the E1 conformation | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SCH28080 | i.e., 2-methyl-8-(phenylmethoxy)imidazo[1,2-a]pyridine-3-acetonitrile | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
K+[side 2] | mutant enzyme E795Q, at pH 7.0 and 37°C | Sus scrofa | |
1.2 | - |
K+[side 2] | wild type enzyme, at pH 7.0 and 37°C | Sus scrofa | |
5.5 | - |
K+[side 2] | mutant enzyme E343Q, at pH 7.0 and 37°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Sus scrofa | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2 mM used in assay conditions | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+[side 1] + K+[side 2] | Sus scrofa | - |
ADP + phosphate + H+[side 2] + K+[side 1] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+[side 1] + K+[side 2] | - |
Sus scrofa | ADP + phosphate + H+[side 2] + K+[side 1] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
H+, K+-ATPase | - |
Sus scrofa |
HKA | - |
Sus scrofa |