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Literature summary for 7.2.2.14 extracted from

  • Schindl, R.; Weghuber, J.; Romanin, C.; Schweyen, R.J.
    Mrs2p forms a high conductance Mg2+ selective channel in mitochondria (2007), Biophys. J., 93, 3872-3883.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.14

Cloned(Commentary)

Cloned (Comment) Organism
expression of HA-tagged wild-type and mutant enzymes in yeast Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
E341D site-directed mutagenesis, the substitution in the loop region of Mrs2p does not influence conductance of the Mg2+ channel Saccharomyces cerevisiae
E341K site-directed mutagenesis, the substitution in the loop region of Mrs2p abolish conductance of the Mg2+ channel Saccharomyces cerevisiae
E342D site-directed mutagenesis, the substitution in the loop region of Mrs2p does not influence conductance of the Mg2+ channel Saccharomyces cerevisiae
E342K site-directed mutagenesis, the substitution in the loop region of Mrs2p abolish conductance of the Mg2+ channel Saccharomyces cerevisiae
additional information construction of the the isogenic mrs2D deletion strain DBY mrs2-1, and the mrs2D/lpe10D double disruptant DBY747 mrs2-2, lpe10-2, Mrs2p coiled-coil mutations results in elevated Mg21 influx into mitochondria Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
cobalt(III)-hexaammine inhibits Mrs2p channels Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, about, recombinant HA-tagged wild-type and mutant enzymes, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Mg2+/out Saccharomyces cerevisiae
-
 ADP + phosphate + Mg2+/in
-
 ?
ATP + H2O + Mg2+/out Saccharomyces cerevisiae DBY747
-
 ADP + phosphate + Mg2+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q02783
-
-
Saccharomyces cerevisiae DBY747 Q02783
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant HA-tagged wild-type and mutant enzymes in yeast, native enzyme partially by preparation of mitochondria Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 Mg2+ transport measurements by patch clamp technique Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Mg2+/out
-
 Saccharomyces cerevisiae ADP + phosphate + Mg2+/in
-
 ?
ATP + H2O + Mg2+/out Mrs2 protein forms a Mg2+-selective channel of high conductance, which has an open probability of about 60% in the absence of Mg2+ at the matrix site, that decreases to about 20% in its presence, the selective channel controls Mg2+ influx into mitochondria by an intrinsic negative feedback mechanism Saccharomyces cerevisiae ADP + phosphate + Mg2+/in
-
 ?
ATP + H2O + Mg2+/out
-
 Saccharomyces cerevisiae DBY747 ADP + phosphate + Mg2+/in
-
 ?
ATP + H2O + Mg2+/out Mrs2 protein forms a Mg2+-selective channel of high conductance, which has an open probability of about 60% in the absence of Mg2+ at the matrix site, that decreases to about 20% in its presence, the selective channel controls Mg2+ influx into mitochondria by an intrinsic negative feedback mechanism Saccharomyces cerevisiae DBY747 ADP + phosphate + Mg2+/in
-
 ?
ATP + H2O + Ni2+/out
-
 Saccharomyces cerevisiae ADP + phosphate + Ni2+/in
-
 ?
ATP + H2O + Ni2+/out
-
 Saccharomyces cerevisiae DBY747 ADP + phosphate + Ni2+/in
-
 ?
additional information with lower conductance, the Mrs2 channel is also permeable for Ni2+, whereas no permeability has been observed for either Ca2+, Mn2+, or Co2+ Saccharomyces cerevisiae ?
-
 ?
additional information with lower conductance, the Mrs2 channel is also permeable for Ni2+, whereas no permeability has been observed for either Ca2+, Mn2+, or Co2+ Saccharomyces cerevisiae DBY747 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, about, recombinant HA-tagged wild-type and mutant enzymes, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
More the enzyme is a member of the CorA-Mrs2-Alr1 superfamily of Mg21 transporters Saccharomyces cerevisiae
Mrs2p
-
 Saccharomyces cerevisiae