Cloned (Comment) | Organism |
---|---|
in pSD5 vector, expression of proteins in Xenopus laevis oocytes | Rhinella marina |
Protein Variants | Comment | Organism |
---|---|---|
C111S | control | Rhinella marina |
G803C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
L802C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
P801C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
T804C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
V805C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MTSET | [2-(trimethylammonium)ethyl]methanethiosulphonate bromide, inhibits mutants G803C, V805C | Rhinella marina | |
Omeprazole | inhibit mutants G803C, T804C, V805C | Rhinella marina | |
Ouabain | g-strophanthin | Rhinella marina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rhinella marina | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
glycosylated beta subunit | Rhinella marina |
110000 | - |
alpha subunit | Rhinella marina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + Na+/out | Rhinella marina | - |
ATP + H2O + Na+/in | - |
? | |
ATP + H2O + K+/in | Rhinella marina | - |
ADP + phosphate + K+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhinella marina | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + Na+/out | - |
Rhinella marina | ATP + H2O + Na+/in | - |
? | |
ATP + H2O + K+/in | - |
Rhinella marina | ADP + phosphate + K+/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | heterodimer, alpha and beta subunit | Rhinella marina |
Synonyms | Comment | Organism |
---|---|---|
Na+,K+-ATPase | - |
Rhinella marina |