Cloned (Comment) | Organism |
---|---|
expression in Xenopus laevis oocyte | Rhinella marina |
in pSD5 vector, expression of proteins in Xenopus laevis oocytes | Rhinella marina |
Protein Variants | Comment | Organism |
---|---|---|
A330C | difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range, negative slope of the I/V curve of the ouabain-sensitive current in the low and positive membrane potential range | Rhinella marina |
A330C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
A338C | difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range | Rhinella marina |
A338C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
C111S | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
C111S | used as control | Rhinella marina |
E334C | negative slope of the I/V curve of the ouabain-sensitive current in the low and positive membrane potential range, K+-activated current with an amplitude half of the oubain-sensitive current in the high negative membrane potential range | Rhinella marina |
E334C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
F323C | small ouabain-sensitive current over the whole membrane potential range, significant K+-induced inward curve mostly at negative membrane potential range | Rhinella marina |
F323C | K+ induced current, I_K in nA, lower than control(negativ) - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
G326C | inhibition by 2-aminoethyl-methanethiosulfonate | Rhinella marina |
G326C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
G335C | identical and strongly voltage-dependent I/V relationship of the K+-induced and ouabain sensitive currents | Rhinella marina |
G335C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
I322C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
I322C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
I325C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
I325C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
I327C | difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range | Rhinella marina |
I327C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
I328C | negative slope of the I/V curve of the ouabain-sensitive current in the low and positive membrane potential range | Rhinella marina |
I328C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
L324C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
L324C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
L336C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
L336C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
L337C | K+ induced current, I_K in nA, lower (negative) than control - ouabain-sensitive current, I_ouab in nA, lower (negative) than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
L344C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
L344C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
N331C | difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range | Rhinella marina |
N331C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
P333C | inhibition by 2-aminoethyl-methanethiosulfonate | Rhinella marina |
P333C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
T339C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower control | Rhinella marina |
T341C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, similar to control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
V321C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
V321C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
V329C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
V329C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
V332C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
V332C | K+ induced current, I_K in nA, similar to control - ouabain-sensitive current, I_ouab in nA, similar to control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
V340C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
V340C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
V342C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminoethyl-methanethiosulfonate | inhibition of mutants F323C, P333C, E334C, G335C, I322C, G326C and A330C | Rhinella marina | |
Ouabain | g-strophanthin | Rhinella marina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rhinella marina | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + Na+/out | Rhinella marina | - |
ATP + H2O + Na+/in | - |
? | |
ATP + H2O + K+/in | Rhinella marina | - |
ADP + phosphate + K+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhinella marina | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + Na+/out | - |
Rhinella marina | ATP + H2O + Na+/in | - |
? | |
ATP + H2O + K+/in | - |
Rhinella marina | ADP + phosphate + K+/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha subunit, main subunit - beta subunit, glycoprotein | Rhinella marina |
Synonyms | Comment | Organism |
---|---|---|
Na,K-ATPase | - |
Rhinella marina |