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Literature summary for 7.2.2.10 extracted from
- Regulation of sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) in turtle muscle and liver during acute exposure to anoxia (2010), J. Exp. Biol., 213, 17-25.
General Stability
| General Stability | Organism |
|---|---|
| the less active enzyme in anoxic turtles features greater stability than the enzyme from normoxic animals. SERCA in liver from anoxic animals is more stable after urea treatment/thermolysin-mediated proteolysis than the normoxic counterpart | Trachemys scripta elegans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| thapsigargin | specific inhibitor, complete inhibition at 50 nM | Trachemys scripta elegans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
x * 120000, SDS-PAGE | Trachemys scripta elegans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trachemys scripta elegans | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | enzyme activity is blunted by protein phosphorylation and enhanced by dephosphorylation. In muscle, incubations promoting protein kinase A or protein kinase G activity reduces enzyme activity in extracts from normoxic turtles by 65% and 83%, respectively, to levels more typical of anoxia-treated animals | Trachemys scripta elegans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Trachemys scripta elegans | - |
| heart | - |
Trachemys scripta elegans | - |
| kidney | lowest protein content and activity in kidney | Trachemys scripta elegans | - |
| liver | - |
Trachemys scripta elegans | - |
| muscle | highest protein content and activity in muscle | Trachemys scripta elegans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Ca2+/out | - |
Trachemys scripta elegans | ADP + phosphate + Ca2+/in | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 120000, SDS-PAGE | Trachemys scripta elegans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sarcoendoplasmic reticulum Ca2+-ATPase | - |
Trachemys scripta elegans |
| SERCA | - |
Trachemys scripta elegans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 8 | anoxic muscle SERCA is relatively stable between pH 6.6 and pH 8.0 | Trachemys scripta elegans |
| 7 | 7.4 | normoxic enzyme shows broad peak of activity greater than anoxic muscle enzyme activity between pH 7.0 and 7.4, but normoxic enzyme activity sharply declines on either side of that range, and is less than that of anoxic muscle enzyme at low pH (6.6-6.8) | Trachemys scripta elegans |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Trachemys scripta elegans | after 20 h of anoxic submergence, enzyme protein levels remain unchanged in skeletal muscle, liver and heart, but decreases by 36% and 24% in kidney and brain, respectively. Vmax decreases by 46% in muscle, 47% in liver and 35% in heart of anoxic turtles, but does not change significantly in other tissues | down |
| Trachemys scripta elegans | stimulation of protein kinase C activity has no effect on SERCA enzyme activity | additional information |
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