Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.2.2.10 extracted from

  • Bonza, M.C.; Luoni, L.
    Plant and animal type 2B Ca2+-ATPases: evidence for a common auto-inhibitory mechanism (2010), FEBS Lett., 584, 4783-4788.
    View publication on PubMed
Search for more literature for 7.2.2.10

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin the fully auto-inhibited enzyme has a very low basal Ca2+-ATPase activity which is stimulated more than 5fold by calmodulin Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae mutant strain K616 devoid of endogenous Ca2+-ATPases Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 x * 115000, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LF79
-
-

Purification (Commentary)

Purification (Comment) Organism
calmodulin-Sepharose column chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0241
-
 recombinant wild type enzyme, in the presence of Ca2+, pH not specified in the publication, at 25°C Arabidopsis thaliana
0.1181
-
 recombinant wild type enzyme, in the presence of Ca2+ and 0.001 mM calmodulin, pH not specified in the publication, at 25°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Ca2+/out
-
 Arabidopsis thaliana ADP + phosphate + Ca2+/in
-
 ?

Subunits

Subunits Comment Organism
? x * 115000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
ACA8
-
 Arabidopsis thaliana
Ca2+-ATPase 8
-
 Arabidopsis thaliana