Literature summary for 7.2.2.10 extracted from

Liu, Y.; Pilankatta, R.; Lewis, D.; Inesi, G.; Tadini-Buoninsegni, F.; Bartolommei, G.; Moncelli, M.R.
High-yield heterologous expression of wild type and mutant Ca2+ ATPase: characterization of Ca(2+) binding sites by charge transfer (2009), J. Mol. Biol., 391, 858-871.

Search for more literature for 7.2.2.10

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	calcium occupancy of site I is required to trigger cooperative binding to site II and catalytic activation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
SERCA1 expression in COS-1 cells targeted extensively to intracellular membranes using an adenoviral vector. Higher transcription and expression are obtained in the presence of a His6 tag at the amino terminus, as compared with a His6 tag at the carboxyl SERCA terminus, or no tag. Optimization of expression method, overview	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D351N	a phosphorylation site mutant, catalytically inactive	Homo sapiens
E309Q	the mutation of Ca2+-binding site II leads to non-cooperative binding of only one Ca2+, and loss of ATPase activation, catalytically inactive mutant	Homo sapiens
E771Q	the mutation of Ca2+-binding site I leads to no Ca2+ binding to either site, catalytically inactive mutant	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Mg2+	competitive Mg2+ binding can occur at Ca2+ binding site I but not at site II	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	charge transfer and current transients measurements, kinetics, overview	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Homo sapiens	-	-
sarcoplasmic reticulum membrane	-	Homo sapiens	33017	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	calcium occupancy of site I is required to trigger cooperative binding to site II and catalytic activation	Homo sapiens
Mg2+	competitive Mg2+ binding can occur at Ca2+ binding site I but not at site II	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Ca2+/cis	Homo sapiens	SERCA1 is responsible for ATPase activity and intermediate reactions, as well as ATP-dependent Ca2+ transport	ADP + phosphate + Ca2+/trans	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isozyme SERCA1	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
skeletal muscle	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Ca2+/cis	SERCA1 is responsible for ATPase activity and intermediate reactions, as well as ATP-dependent Ca2+ transport	Homo sapiens	ADP + phosphate + Ca2+/trans	-	?
ATP + H2O + Ca2+/cis	analysis of Ca2+ binding at the two specific transmembrane sites using detergent- and ATP-free enzyme, yielding cooperative isotherms. Calcium occupancy of site I is required to trigger cooperative binding to site II and catalytic activation. Charge transfer and current transients measurements, overview	Homo sapiens	ADP + phosphate + Ca2+/trans	-	?

Synonyms

Synonyms	Comment	Organism
Ca2+ ATPase	-	Homo sapiens
sarco(endo)plasmic reticulum ATPase	-	Homo sapiens
SERCA1	-	Homo sapiens

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Release 2023.1 (January 2023)