Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
high-resolution crystal structures of cytochrome b561 from Arabidopsis thaliana in both substrate-free and substrate-bound states is reported. Cyt b561 forms a homodimer, with each protomer consisting of six transmembrane helices and two heme groups | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
F105W/H106E | mutations on the noncytoplasmic side only still allows the oxidized Cyt b561 to be reduced by ascorbate | Arabidopsis thaliana |
K81A/R150A | mutations on the cytoplasmic side only still allows the oxidized Cyt b561 to be reduced by ascorbate | Arabidopsis thaliana |
K81A/R150A/F105W/H106E | mutant carrying ascorbate-binding mutations on both cytoplasmic and noncytoplasmic sides, completely loses its ability to be reduced by ascorbate | Arabidopsis thaliana |
Y115W | mutations on the noncytoplasmic side only still allows the oxidized Cyt b561 to be reduced by ascorbate | Arabidopsis thaliana |
Y140W | mutations on the cytoplasmic side only still allows the oxidized Cyt b561 to be reduced by ascorbate | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SWS1 | - |
- |
Purification (Comment) | Organism |
---|---|
purified by affinity chromatography and gel filtration | Arabidopsis thaliana |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
Cyt b561 | - |
Arabidopsis thaliana |
cytochrome b561 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | oxidized Cyt b561 is reduced by ascorbate | Arabidopsis thaliana |