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Literature summary for 7.1.3.1 extracted from
- Proton-pyrophosphatase and polyphosphate in acidocalcisome-like vesicles from oocytes and eggs of Periplaneta americana (2009), Insect Biochem. Mol. Biol., 39, 198-206.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Periplaneta americana |  |
| Imidodiphosphate | when diphosphate concentration in the medium is adjusted to 0.05 mM, the enzyme is totally inhibited by 0.5 mM imidodiphosphate, the same concentration of imidodiphosphate only decreases the enzyme activity by 70% and 50% when diphosphate concentration in the medium is increased to 0.15 mM and 0.3 mM, respectively | Periplaneta americana | |
| additional information | not inhibited by K+ | Periplaneta americana | |
| Sodium fluoride | - |
Periplaneta americana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| acidocalcisome | commonly present in membranes of acidocalcisomes | Periplaneta americana | 20022 | - |
| vesicle | preferentially located in the small vesicles | Periplaneta americana | 31982 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, maximum activity is obtained in the presence of 5 mM Mg2+, no diphosphate hydrolysis is detected in the absence of Mg2+ | Periplaneta americana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
SDS-PAGE | Periplaneta americana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Periplaneta americana | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Periplaneta americana | - |
| oocyte | - |
Periplaneta americana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O + H+[side 1] | - |
Periplaneta americana | 2 phosphate + H+[side 2] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| H+-PPase | - |
Periplaneta americana |
| proton-pyrophosphatase | electrogenic proton-pump that couples diphosphate hydrolysis to the active transport of protons across membranes | Periplaneta americana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity optimum at pH around 7.0 | Periplaneta americana |
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Release 2023.1 (January 2023)
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