Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C43(DE3) | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
D204H/R206L | 90% loss in proton translocating but only 50% loss in hydrolytic activity | Rhodospirillum rubrum |
D217A | almost complete loss of hydrolytic and proton translocating activity, less Mg2+ required for activity | Rhodospirillum rubrum |
D217H | almost complete loss of hydrolytic and proton translocating activity, less Mg2+ required for activity | Rhodospirillum rubrum |
H372A | slight loss of activity | Rhodospirillum rubrum |
H372A/H702V | slight loss of activity | Rhodospirillum rubrum |
H469R | almost complete loss of hydrolytic and proton translocating activity | Rhodospirillum rubrum |
H652A | slight loss of activity | Rhodospirillum rubrum |
H661A | about 50% loss of activity | Rhodospirillum rubrum |
H702V | slight loss of activity | Rhodospirillum rubrum |
General Stability | Organism |
---|---|
Mg2+/diphosphate protects against inhibition by all inhibitors in the study | Rhodospirillum rubrum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-bromophenacyl bromide | - |
Rhodospirillum rubrum | |
diethylpyrocarbonate | - |
Rhodospirillum rubrum | |
Fluorescein 5'-isothiocyanate | - |
Rhodospirillum rubrum | |
N,N'-dicyclohexyl-carbodiimide | - |
Rhodospirillum rubrum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Rhodospirillum rubrum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rhodospirillum rubrum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | Rhodospirillum rubrum | important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation | 2 phosphate + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | - |
Rhodospirillum rubrum | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation | Rhodospirillum rubrum | 2 phosphate + H+[side 2] | - |
? |