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Literature summary for 7.1.2.2 extracted from

  • Soontharapirakkul, K.; Promden, W.; Yamada, N.; Kageyama, H.; Incharoensakdi, A.; Iwamoto-Kihara, A.; Takabe, T.
    Halotolerant cyanobacterium Aphanothece halophytica contains an Na+-dependent F1F0-ATP synthase with a potential role in salt-stress tolerance (2011), J. Biol. Chem., 286, 10169-10176.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli mutant DK8 deficient in ATP synthase and in Synechococcus sp. strain PCC 7942 Aphanothece halophytica

Inhibitors

Inhibitors Comment Organism Structure
1,3-dicyclohexyl carbodiimide
-
 Aphanothece halophytica
monensin 70% inhibition at 0.1 mM; 70% inhibition at 0.1 mM; 70% inhibition at 0.1 mM Aphanothece halophytica
additional information not inhibited by carbonyl cyanide m-chlorophenyl hydrazine; not inhibited by carbonyl cyanide m-chlorophenyl hydrazine; not inhibited by carbonyl cyanide m-chlorophenyl hydrazine Aphanothece halophytica
N,NĀ’-dicyclohexylcarbodiimide
-
 Aphanothece halophytica
Tributyltin chloride 80% inhibition at 0.1 mM; 80% inhibition at 0.1 mM; 80% inhibition at 0.1 mM Aphanothece halophytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 ATP apparent value, recombinant enzyme, in 20 mM Tris-HCl, pH 7.6, 5mM MgCl2, 10 mM NaCl, temperature not specified in the publication Aphanothece halophytica
3
-
 Na+/in apparent value, recombinant enzyme, in 20 mM Tris-HCl, pH 7.6, 5mM MgCl2, 10 mM NaCl, temperature not specified in the publication Aphanothece halophytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
 Aphanothece halophytica
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ ApNa+-ATPase has a putative Na+-binding site in subunit epsilon. The Na+ ion protects the inhibition of Na+-ATPase by N,NĀ’-dicyclohexylcarbodiimide Aphanothece halophytica
Na+ the Na+ ion protects the inhibition of Na+-ATPase by N,NĀ’-dicyclohexylcarbodiimide Aphanothece halophytica

Organism

Organism UniProt Comment Textmining
Aphanothece halophytica F2Z9M7 epsilon subunit
-
Aphanothece halophytica F2Z9M8 I subunit
-
Aphanothece halophytica F2Z9N4 gamma subunit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Na+/in
-
 Aphanothece halophytica ADP + phosphate + Na+/out
-
 ?

Synonyms

Synonyms Comment Organism
ApNa+-ATPase
-
 Aphanothece halophytica
Na+-dependent F1F0-ATP synthase
-
 Aphanothece halophytica

General Information

General Information Comment Organism
physiological function Na+-dependent F1F0-ATP synthase in the cytoplasmic membrane plays a potential role in salt-stress tolerance Aphanothece halophytica