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Literature summary for 7.1.2.2 extracted from

  • Zuo, J.; Vergara, S.; Kohno, S.; Holliday, L.S.
    Biochemical and functional characterization of the actin-binding activity of the B subunit of yeast vacuolar H+-ATPase (2008), J. Exp. Biol., 211, 1102-1108.
    View publication on PubMed
Search for more literature for 7.1.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant V-ATPase B subunits as maltose-binding protein fusion proteins in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information mutations in the profilin-like region, actin-binding domain, of the B subunit reduced or eliminated the actin-binding activity. Mutants assemble properly with endogenous yeast subunits when expressed in B subunit-null yeast, and bafilomycin-sensitive ATPase activity is not significantly different from yeast transformed with wild-type B subunit. Yeast containing the mutant subunits grow as well at pH 7.5 as wild-type. Mutant B subunits are more sensitive to cycloheximide and wortmannin than those transformed with wild-type B subunits Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuolar membrane
-
 Saccharomyces cerevisiae 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in Saccharomyces cerevisiae
-
 ADP + phosphate + H+/out
-
 ?
additional information Saccharomyces cerevisiae bacterially expressed B subunit from the yeast Saccharomyces cerevisiae binds actin filaments. Actin-binding activity confers on the B subunit of yeast a function that is distinct from its role in the enzymatic activity of the proton pump ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant V-ATPase B subunits as maltose-binding protein fusion proteins from Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Saccharomyces cerevisiae ADP + phosphate + H+/out
-
 ?
additional information bacterially expressed B subunit from the yeast Saccharomyces cerevisiae binds actin filaments. Actin-binding activity confers on the B subunit of yeast a function that is distinct from its role in the enzymatic activity of the proton pump Saccharomyces cerevisiae ?
-
 ?
additional information the enzyme B subunit binds purified polymerized actin from rabbit muscle Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
V-ATPase
-
 Saccharomyces cerevisiae
vacuolar H+-ATPase
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.75
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae