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Literature summary for 7.1.2.2 extracted from

  • McCarty, R.E.
    ATP synthase of chloroplast thylakoid membranes: A more in depth characterization of its ATPase activity (2005), J. Bioenerg. Biomembr., 37, 289-299.
    View publication on PubMed
Search for more literature for 7.1.2.2

Activating Compound

Activating Compound Comment Organism Structure
light promotes ATP hydrolysis Spinacia oleracea
sulfite 10 mM stimulates both proton uptake and ATP hydrolysis of Mg2+-ATPase activity of trypsin-treated thylakoids Spinacia oleracea
Trypsin elicits higher rates of ATPase activity, Mg2+-ATPase activity of trypsinized thylakoids is only partially inhibited by the uncouplers Spinacia oleracea

Application

Application Comment Organism
additional information cleavage of the gamma subunit of the ATP synthase by trypsin prevents inhibition of ATPase activity by the sigma subunit, but only partially overcomes Mg2+-ADP inhibition during assay Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
ADP incubation prior to assay abolishes Mg2+-ATPase activity of reduced thylakoids, has no effect on Mg2+-ATPase activity of trypsinized thylakoids Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-
thylakoid membrane
-
 Spinacia oleracea 42651
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ contributes to significant ATPase activity in trypsin-treated membranes, whereas Ca2+-ATPase activity of reduced thylakoids is very low Spinacia oleracea
Mg2+
-
 Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + H+
-
 Spinacia oleracea ATP + H2O
-
 r
ATP + H2O + H+/in
-
 Spinacia oleracea ADP + phosphate + H+/out
-
 r

Synonyms

Synonyms Comment Organism
chloroplast ATP synthase
-
 Spinacia oleracea