Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N,N'-dicyclohexylcarbodiimide | 0.1 mM increases ATPase activity by 20% | Acidithiobacillus ferrooxidans | |
sulfite | 50 mM stimulates 4fold | Acidithiobacillus ferrooxidans |
Cloned (Comment) | Organism |
---|---|
strain ATCC3020 in Escherichia coli-derived in vitro transcription-translation system | Acidithiobacillus ferrooxidans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 2 mM inhibits 91% ATPase activity | Acidithiobacillus ferrooxidans | |
Cl- | - |
Acidithiobacillus ferrooxidans | |
Cu2+ | 1 mM reduces ATPase activity 99% in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans | |
Mn2+ | 1 mM reduces ATPase activity 50% in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans | |
additional information | relatively resistant to vanadate, N,N'-dicyclohexylcarbodiimide and nitrate, 1 mM Fe3+, Ca2+ and Na+ do not affect ATPase activity in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans | |
NaCl | inhibits ATPase activity | Acidithiobacillus ferrooxidans | |
Ni2+ | 1 mM reduces ATPase activity 47% in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans | |
sulfate | slightly inhibits | Acidithiobacillus ferrooxidans | |
Zn2+ | 1 mM reduces ATPase activity 80% in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
ATP | estimated at pH 8.5 at 40°C | Acidithiobacillus ferrooxidans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Acidithiobacillus ferrooxidans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Acidithiobacillus ferrooxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
strain NASF-1, strain ATCC33020 | - |
Purification (Comment) | Organism |
---|---|
51fold by chromatographies, purified ATPase does not contain the F0-portion | Acidithiobacillus ferrooxidans |
Storage Stability | Organism |
---|---|
4°C, Tris-SO4 buffer, pH 8.5, 8 days | Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Acidithiobacillus ferrooxidans | ADP + phosphate + H+/out | - |
? | |
GTP + H2O + H+/in | - |
Acidithiobacillus ferrooxidans | GDP + phosphate + H+/out | - |
? | |
ITP + H2O + H+/in | - |
Acidithiobacillus ferrooxidans | IDP + phosphate + H+/out | - |
? | |
additional information | no hydrolysis of UTP nor ADP | Acidithiobacillus ferrooxidans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 33000, + x * F1delta, 20000, + x * F1epsilon, 18000, SDS-PAGE of strain NASF-1, x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 30000, + x * F1delta, 23000, + x * F1epsilon, 14000, SDS-PAGE of strain ATCC33020, x * F1alpha, 55500 + x * F1beta, 50500, + x * F1gamma, 33100, + x * F1delta, 19200, + x * F1epsilon, 15100, sequence analysis of strain NASF-1 | Acidithiobacillus ferrooxidans |
Synonyms | Comment | Organism |
---|---|---|
F1-ATPase | - |
Acidithiobacillus ferrooxidans |
F1F0-ATPase | - |
Acidithiobacillus ferrooxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Acidithiobacillus ferrooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Acidithiobacillus ferrooxidans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | ATPase activity decreases markedly above or below this range | Acidithiobacillus ferrooxidans |