Literature summary for 7.1.1.1 extracted from

Bizouarn, T.; Stilwell, S.; Venning, J.; Cotton, N.P.J.; Jackson, J.B.
The pH dependences of reactions catalyzed by the complete proton-translocating transhydrogenase from Rhodospirillum rubrum, and by the complex formed from its recombinant nucleotide-binding domains (1997), Biochim. Biophys. Acta, 1322, 19-32.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Rhodospirillum rubrum	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14900	-	1 * 47800 + 1 * 14900, SDS-PAGE	Rhodospirillum rubrum
47800	-	1 * 47800 + 1 * 14900, SDS-PAGE	Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NADPH + NAD+ + H+[side 1]	Rhodospirillum rubrum	-	NADP+ + NADH + H+[side 2]	-	r

Organism

Organism	UniProt	Comment	Textmining
Rhodospirillum rubrum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography and Mono Q column chromatography	Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADPH + NAD+ + H+[side 1]	-	Rhodospirillum rubrum	NADP+ + NADH + H+[side 2]	-	r
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]	-	Rhodospirillum rubrum	NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]	-	?
thio-NADPH + NAD+ + H+[side 1]	-	Rhodospirillum rubrum	thio-NADP+ + NADH + H+[side 2]	-	?

Subunits

Subunits	Comment	Organism
heterodimer	1 * 47800 + 1 * 14900, SDS-PAGE	Rhodospirillum rubrum

Synonyms

Synonyms	Comment	Organism
proton-translocating transhydrogenase	-	Rhodospirillum rubrum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.3	-	pH optimum for the reduction of thio-NADP+ by NADH	Rhodospirillum rubrum
7.1	-	pH optimum for the reduction of oxidized acetyl pyridine adenine dinucleotide by NADPH	Rhodospirillum rubrum

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Release 2023.1 (January 2023)