Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
membrane | the enzyme is composed of three components. The dI and dIII components, which house the binding site for NAD(H) and NADP(H), respectively, are peripheral to the membrane, and dII spans the membrane | Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+/out + NADH + NADP+ | Escherichia coli | the enzyme couples the redox reaction between NAD(H) and NADP(H) to the transport of protons across a membrane | H+/in + NAD+ + NADPH | - |
? | |
NADPH + NAD+ + H+[side 1] | Escherichia coli | - |
NADP+ + NADH + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
purified enzyme, at pH 7.0 and 20°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+/out + NADH + NADP+ | the enzyme couples the redox reaction between NAD(H) and NADP(H) to the transport of protons across a membrane | Escherichia coli | H+/in + NAD+ + NADPH | - |
? | |
NADPH + NAD+ + H+[side 1] | - |
Escherichia coli | NADP+ + NADH + H+[side 2] | - |
? | |
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Escherichia coli | NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
r |
Synonyms | Comment | Organism |
---|---|---|
proton-translocating transhydrogenase | - |
Escherichia coli |