Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | with 30 mM (NH4)2SO4, the activity is maximal | Haemophilus influenzae | |
KCl | activation by KCl is observed at (NH4)2SO4 concentrations below 30 mM, in the absence of (NH4)2SO4 ligase activity is activated by KCl over 0-100 mM, and at 100 mM KCl, further activation occurs at up to 12 mM (NH4)2SO4 | Haemophilus influenzae |
Cloned (Comment) | Organism |
---|---|
- |
Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | (NH4)2SO4 concentrations higher than 30 mM are inhibitory. At 100 mM KCl, concentrations of (NH4)2SO4 above 12 mM are inhibitory | Haemophilus influenzae | |
1,N6-etheno-NAD+ | - |
Haemophilus influenzae | |
KCl | inhibition by KCl occurs at greater than 30 mM (NH4)2SO | Haemophilus influenzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, maximal activity occurs with 10-20 mM MgCl2 in the presence of 30 mM each of KCl and (NH4)2SO4 | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Haemophilus influenzae | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent DNA ligase | - |
Haemophilus influenzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Haemophilus influenzae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.054 | - |
pH and temperature not specified in the publication | Haemophilus influenzae | 1,N6-etheno-NAD+ |