Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
F417Y | beta-subunit mutant retains activity, kcat/Km increased compared to wild-type, kcat (3-methylcrotonoyl-CoA) increased compared to wild-type, Km (3-methylcrotonoyl-CoA) increased compared to wild-type | Pseudomonas aeruginosa |
F417Y/G423A | beta-subunit mutant retains activity, kcat/Km similar to wild-type, kcat (3-methylcrotonoyl-CoA) increased compared to wild-type, Km (3-methylcrotonoyl-CoA) increased compared to wild-type | Pseudomonas aeruginosa |
G423A | beta-subunit mutant retains activity, kcat/Km decreased compared to wild-type, kcat (3-methylcrotonoyl-CoA) decreased compared to wild-type, Km (3-methylcrotonoyl-CoA) slightly decreased compared to wild-type | Pseudomonas aeruginosa |
R51A | beta-subunit mutant retains activity, kcat/Km decreased compared to wild-type, kcat (3-methylcrotonoyl-CoA) decreased compared to wild-type, Km (3-methylcrotonoyl-CoA) slightly increased compared to wild-type | Pseudomonas aeruginosa |
S187A | beta-subunit mutant shows no enzymatic activity | Pseudomonas aeruginosa |
Y422D | beta-subunit mutant shows no enzymatic activity | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.179 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
0.216 | - |
3-methylcrotonoyl-CoA | wild-type, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
0.309 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant R51A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
0.315 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y/G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
0.514 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y, pH and temperature not specified in the publication | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I299 and Q9I297 | Q9I299, alpha-subunit and Q9I297, beta-subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Pseudomonas aeruginosa | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-methylcrotonyl-coenzyme A | - |
Pseudomonas aeruginosa |
Pa-MCCase | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
3-methylcrotonoyl-CoA | beta-subunit beta-subunit mutant R51A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
0.66 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
0.96 | - |
3-methylcrotonoyl-CoA | wild-type, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
1.45 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y/G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
2.76 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y, pH and temperature not specified in the publication | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.39 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant R51A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
3.68 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
4.4 | - |
3-methylcrotonoyl-CoA | wild-type, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
4.6 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y/G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
5.36 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y, pH and temperature not specified in the publication | Pseudomonas aeruginosa |