Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-2-methyl-2-butenoyl-CoA | weak | Zea mays | |
acetoacetyl-CoA | - |
Zea mays | |
ATP | - |
Zea mays | |
butanoyl-CoA | weak | Zea mays | |
isobutanoyl-CoA | weak | Zea mays | |
Li+ | slight inhibition | Zea mays | |
n-hexanoyl-CoA | weak | Zea mays | |
Na+ | slight inhibition | Zea mays | |
p-hydroxymercuribenzoate | - |
Zea mays | |
pentanoyl-CoA | weak | Zea mays | |
Phenylglyoxal | 3-methylcrotonoyl-CoA or ATP protects | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
3-methylcrotonoyl-CoA | - |
Zea mays | |
0.02 | - |
ATP | - |
Zea mays | |
0.11 | - |
crotonoyl-CoA | - |
Zea mays | |
0.8 | - |
HCO3- | - |
Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates with 57% of the efficiency relative to Mg2+ | Zea mays | |
Cs+ | monovalent cations activate, K+, Cs+, Rb+ or NH4+ | Zea mays | |
K+ | monovalent cations activate, K+, Cs+, Rb+ or NH4+ | Zea mays | |
Mg2+ | maximal activity at 2 mM Mg2+, at ATP concentrations 0.025 mM, 0.05 mM or 1 mM | Zea mays | |
Mg2+ | divalent cation required, Mg2+ is the best activator | Zea mays | |
Mn2+ | activates with 77% of the efficiency relative to Mg2+ | Zea mays | |
NH4+ | monovalent cations activate, K+, Cs+, Rb+ or NH4+ | Zea mays | |
Rb+ | monovalent cations activate, K+, Cs+, Rb+ or NH4+. Maximal activity obtained with Rb+ | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58500 | - |
6 * 58500 + 6 * 80000, SDS-PAGE | Zea mays |
80000 | - |
6 * 58500 + 6 * 80000, SDS-PAGE | Zea mays |
835000 | - |
PAGE, gel filtration | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 3-methylcrotonoyl-CoA + HCO3- + H+ = ADP + phosphate + 3-methylglutaconyl-CoA | ATP and HCO3- bind sequentially to the enzyme. ATP and 3-methylcrotonoyl-CoA bind in ping-pong fashion | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.206 | - |
- |
Zea mays |
Storage Stability | Organism |
---|---|
-20°C, for at least 3 months without loss of activity | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Zea mays | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
ATP + acetoacetyl-CoA + HCO3- | acetoacetyl-CoA inhibits, no activity as substrate | Zea mays | ? | - |
? | |
ATP + crotonoyl-CoA + HCO3- | - |
Zea mays | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 6 * 58500 + 6 * 80000, SDS-PAGE | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 10.1 | 6.8: more than 70% of maximal activity, 9.5: more than 70% of maximal activity, 10.1: 33% of maximal activity | Zea mays |