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Literature summary for 6.4.1.1 extracted from

  • Choi, P.; Jo, J.; Lin, Y.; Lin, M.; Chou, C.; Dietrich, L.; Tong, L.
    A distinct holoenzyme organization for two-subunit pyruvate carboxylase (2016), Nat. Commun., 7, 12713 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.4.1.1

Cloned(Commentary)

Cloned (Comment) Organism
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 Pseudomonas aeruginosa
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 Methylobacillus flagellatus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of biotin carboxylase domain deletion mutant and of mutant K419A/E421A/E422A reveal an alpha2beta4 stoichiometry Methylobacillus flagellatus

Protein Variants

Protein Variants Comment Organism
A465R mutation in the alpha subunit, abolishes the formation of the holoenzyme, catalytically inactive Methylobacillus flagellatus
A55T mutation in the beta subunit, reduces activity by 50fold and interferes with biotin binding to the active site,mutant exhibits growth on pyruvate similar to the wild type Pseudomonas aeruginosa
D502A/E507A mutation in the alpha subunit, mutant forms a stable holoenzyme, about 40% of wild-type activity Methylobacillus flagellatus
H476A/E478A mutation in the alpha subunit, mutant forms a stable holoenzyme and is fully active Methylobacillus flagellatus
H476A/E478A/D502A/E507A mutation in the alpha subunit, abolishes the formation of the holoenzyme, catalytically inactive Methylobacillus flagellatus
K419A/E421A/E422A mutations bin the beta subunit designed to reduce the surface entropy. The mutations have no effect on the catalytic activity of the enzyme Methylobacillus flagellatus
K451stop mutation in alpha subunit, disrupts holoenzyme formation Pseudomonas aeruginosa
K572A mutation in the beta subunit, abolishes biotinylation and leads to growth defects in pyruvate that are similar to that of a gene deletion mutant Pseudomonas aeruginosa
Q452stop mutation in the alpha subunit, abolishes the formation of the holoenzyme Methylobacillus flagellatus
R401E mutation in the alpha subunit, mutant forms a stable holoenzyme, about 50% of wild-type activity Methylobacillus flagellatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
340000
-
 analytical ultracentrifugation Methylobacillus flagellatus

Organism

Organism UniProt Comment Textmining
Methylobacillus flagellatus Q1H157
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Methylobacillus flagellatus DSM 6875 Q1H157
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Pseudomonas aeruginosa
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-
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Synonyms

Synonyms Comment Organism
Mfla_1512
-
 Methylobacillus flagellatus

General Information

General Information Comment Organism
physiological function a gene deletion mutant shows similar morphologies to those of the wild-typewhen succinate is supplied as the carbon source. When glucose or pyruvate is provided as the carbon source, the mutation prevents growth. In the colony morphology assay, the wild type initially produces a smooth colony, which forms wrinkle structures in the centre starting on day 3. The deletion mutant and K572A colonies begin to wrinkle on day 2 and exhibit a flatter morphology with several exaggerated wrinkles in a spoke formation Pseudomonas aeruginosa