Protein Variants | Comment | Organism |
---|---|---|
K119Q | no catalytic acitivy for pyruvate decarboxylation, or oxaloacetate decarboxylation | Rhizobium etli |
K718Q | 2.9% of wild-type activity for pyruvate carboxylation, 14% for full reverse reaction, 7.2% for oxaloacetate decarboxylation in presence of oxamate | Rhizobium etli |
Q844L/S885A | 13% of wild-type activity for pyruvate carboxylation, 53% for full reverse reaction, 4.7% for oxaloacetate decarboxylation in presence of oxamate | Rhizobium etli |
T882A | no catalytic activity for reactions involving the carboxyl transferase domain. 7- and 3.5fold increases in activity, as compared to that of the wild-type enzyme, for the ADP phosphorylation and bicarbonate-dependent ATPase reactions, respectively. Partial inhibition of the T882A-catalyzed biotin carboxylase domain reactions by oxamate and pyruvate | Rhizobium etli |
T882C | 7.1% of wild-type activity for pyruvate carboxylation, 20% for full reverse reaction, 11% for oxaloacetate decarboxylation in presence of oxamate | Rhizobium etli |
T882S | 21% of wild-type activity for pyruvate carboxylation, 51% for full reverse reaction, 30% for oxaloacetate decarboxylation in presence of oxamate | Rhizobium etli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxamate | substrate inhibition | Rhizobium etli | |
pyruvate | substrate inhibition | Rhizobium etli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
pyruvate | ATPase reaction, wild-type, pH 7.5, 25°C | Rhizobium etli | |
4.1 | - |
oxamate | ATPase reaction, wild-type, pH 7.5, 25°C | Rhizobium etli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium etli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + pyruvate + HCO3- + H+ = ADP + phosphate + oxaloacetate | catalytic mechanism involves the decarboxylation of carboxybiotin and removal of a proton from Thr882 by the resulting biotin enolate with either a concerted or subsequent transfer of a proton from pyruvate to Thr882. The resulting enolpyruvate then reacts with CO2 to form oxaloacetate and complete the reaction | Rhizobium etli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxamate + HCO3- + H+ | - |
Rhizobium etli | ADP + phosphate + ? | - |
? | |
ATP + pyruvate + HCO3- + H+ | - |
Rhizobium etli | ADP + phosphate + oxaloacetate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
pyruvate | ATPase reaction, wild-type, pH 7.5, 25°C | Rhizobium etli | |
10.1 | - |
oxamate | ATPase reaction, wild-type, pH 7.5, 25°C | Rhizobium etli |