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Literature summary for 6.3.5.1 extracted from

  • Yu, C.K.; Dietrich, L.S.
    Purification and properties of yeast nicotinamide adenine dinucleotide synthetase (1972), J. Biol. Chem., 247, 4794-4802.
    View publication on PubMed

General Stability

General Stability Organism
repeated freezing and thawing accelerates inactivation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine
-
Saccharomyces cerevisiae
azaserine progressively inhibits glutamine-dependent activity. With NH4+ as amide donor there is an initial 20% inhibition, then the NH4+-dependent activity remains constant Saccharomyces cerevisiae
diisopropyl fluorophosphate
-
Saccharomyces cerevisiae
Mn2+ can partially replace Mg2+ at 1 mM. At 5 mM inhibition of NAD+ formation Saccharomyces cerevisiae
p-chloromercuribenzoate glutamine-dependent and NH4+-dependent activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
ATP pH 7.6 Saccharomyces cerevisiae
0.19
-
deamido-NAD+ pH 7.6 Saccharomyces cerevisiae
5
-
Gln pH 7.6 Saccharomyces cerevisiae
6.4
-
NH4+ pH 8.6 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+, at 5 mM 30% of the activity relative to Mg2+ Saccharomyces cerevisiae
K+ required Saccharomyces cerevisiae
Mg2+ required Saccharomyces cerevisiae
Mn2+ can partially replace Mg2+ at 1 mM. At 5 mM inhibition of NAD+ formation Saccharomyces cerevisiae
NH4+ as effective as K+ in stimulating activity Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
630000
-
high speed equilibrium ultracentrifugation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
4Ā°C, 0.2 mg per ml, 10 d, 60% loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + Gln + hydroxylamine
-
Saccharomyces cerevisiae AMP + diphosphate + hydroxamate analog + ?
-
?
ATP + deamido-NAD+ + L-Gln
-
Saccharomyces cerevisiae AMP + diphosphate + NAD+ + L-Glu
-
?
ATP + deamido-NAD+ + NH4+
-
Saccharomyces cerevisiae AMP + diphosphate + NAD+
-
?
Gln + hydroxylamine
-
Saccharomyces cerevisiae AMP + diphosphate + hydroxamate analog + ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information turnover number: 1260, NAD+ production Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 8.4 broad peak of activity with a maximum around 7.6, with Gln as the amide donor Saccharomyces cerevisiae
8.4 8.8 with NH4+ as amide donor Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7.8 9.3 7.8: about 40% of maximal activity, 9.3: about 50% of maximal activity, with NH4+ as amide donor Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 overnight, no loss of activity Saccharomyces cerevisiae