BRENDA - Enzyme Database
show all sequences of 6.3.4.22

Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea

Ikeuchi, Y.; Kimura, S.; Numata, T.; Nakamura, D.; Yokogawa, T.; Ogata, T.; Wada, T.; Suzuki, T.; Suzuki, T.; Nat. Chem. Biol. 6, 277-282 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Archaeoglobus fulgidus
Engineering
Amino acid exchange
Commentary
Organism
C352A/C355A
almost no 2-agmatinylcytidine formation is observed
Archaeoglobus fulgidus
G218A
mutation reduces activity
Archaeoglobus fulgidus
R140/G141A
mutation completely abolishes activity
Archaeoglobus fulgidus
R164A
mutation reduces activity
Archaeoglobus fulgidus
R217A
mutation reduces activity
Archaeoglobus fulgidus
T248A/D249A
mutation reduces activity
Archaeoglobus fulgidus
Y163A
mutation reduces activity
Archaeoglobus fulgidus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + agmatine + [tRNAIle2]-cytidine34
Archaeoglobus fulgidus
-
[tRNAIle2]-2-agmatinylcytidine34 + ?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Archaeoglobus fulgidus
O28025
-
-
Pyrococcus horikoshii
O59476
-
-
Purification (Commentary)
Commentary
Organism
-
Archaeoglobus fulgidus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + agmatine + [tRNAIle2]-cytidine34
-
705848
Archaeoglobus fulgidus
[tRNAIle2]-2-agmatinylcytidine34 + ?
-
-
-
-
ATP + agmatine + [tRNAIle2]-cytidine34
-
705848
Archaeoglobus fulgidus
[tRNAIle2]-2-agmatinylcytidine34 + ?
-
-
-
?
ATP + agmatine + [tRNAIle2]-cytidine34
-
705848
Pyrococcus horikoshii
[tRNAIle2]-2-agmatinylcytidine34 + ?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Archaeoglobus fulgidus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C352A/C355A
almost no 2-agmatinylcytidine formation is observed
Archaeoglobus fulgidus
G218A
mutation reduces activity
Archaeoglobus fulgidus
R140/G141A
mutation completely abolishes activity
Archaeoglobus fulgidus
R164A
mutation reduces activity
Archaeoglobus fulgidus
R217A
mutation reduces activity
Archaeoglobus fulgidus
T248A/D249A
mutation reduces activity
Archaeoglobus fulgidus
Y163A
mutation reduces activity
Archaeoglobus fulgidus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + agmatine + [tRNAIle2]-cytidine34
Archaeoglobus fulgidus
-
[tRNAIle2]-2-agmatinylcytidine34 + ?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Archaeoglobus fulgidus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + agmatine + [tRNAIle2]-cytidine34
-
705848
Archaeoglobus fulgidus
[tRNAIle2]-2-agmatinylcytidine34 + ?
-
-
-
-
ATP + agmatine + [tRNAIle2]-cytidine34
-
705848
Archaeoglobus fulgidus
[tRNAIle2]-2-agmatinylcytidine34 + ?
-
-
-
?
ATP + agmatine + [tRNAIle2]-cytidine34
-
705848
Pyrococcus horikoshii
[tRNAIle2]-2-agmatinylcytidine34 + ?
-
-
-
-
General Information
General Information
Commentary
Organism
physiological function
modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine
Archaeoglobus fulgidus
physiological function
modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine
Pyrococcus horikoshii
General Information (protein specific)
General Information
Commentary
Organism
physiological function
modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine
Archaeoglobus fulgidus
physiological function
modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine
Pyrococcus horikoshii
Other publictions for EC 6.3.4.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718503
Osawa
Crystallization and preliminar ...
Archaeoglobus fulgidus
Acta Crystallogr. Sect. F
67
1414-1416
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
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1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720531
Terasaka
Biogenesis of 2-agmatinylcytid ...
Archaeoglobus fulgidus
Nat. Struct. Mol. Biol.
18
1268-1274
2011
-
-
-
-
21
-
2
3
-
2
-
1
-
1
-
1
1
-
-
-
-
-
2
-
1
-
-
3
1
-
-
-
-
-
2
-
-
-
-
-
21
-
2
2
-
3
-
2
-
1
-
-
1
1
-
-
-
-
2
-
1
-
-
3
1
-
-
-
-
-
-
-
3
3
720532
Osawa
Structural basis of tRNA agmat ...
Archaeoglobus fulgidus
Nat. Struct. Mol. Biol.
18
1275-1280
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
1
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-
-
-
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705848
Ikeuchi
Agmatine-conjugated cytidine i ...
Archaeoglobus fulgidus, Pyrococcus horikoshii
Nat. Chem. Biol.
6
277-282
2010
-
-
1
-
7
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-