Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
UDP-N-acetylmuramoyl-L-Ala-D-Glu | pH 8.6, 37°C | Staphylococcus aureus | |
0.53 | - |
ATP | pH 8.6, 37°C | Staphylococcus aureus | |
0.55 | - |
L-Lys | pH 8.6, 37°C | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal concentration 15 mM | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys | - |
Staphylococcus aureus | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys | - |
? | |
additional information | enzyme is specific for L-lysine and does not accept meso-diaminopimelic acid as substrate. The inverse and strict substrate specificities of the two MurE orthologues are responsible for the presence of exclusively mesodiaminopimelic acid and L-lysine at the third position of the peptide in the peptidoglycans of Escherichia coli and Staphylococcus aureus, respectively | Staphylococcus aureus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 9.2 | - |
Staphylococcus aureus |