Crystallization (Comment) | Organism |
---|---|
apo, ADP-bound, ATP-bound, and AMPPNP-bound enzyme, sitting drop vapor diffusion method, mixing of 0.0025 ml of protein solution with 00.0025 ml of reservoir solution containing 15% w/v PEG 4000, 0.1 M Tris, pH 8.5, and 0.2 M MgCl2 with 0.3 M dimethylethyl-(3-sulfopropyl)-ammonium, 14°C, 24 h, ligand-bound enzyme structures by soaking apoenzyme crystals in each nucleotide-containing reservoir solution, X-ray diffraction structure determination and analysis at 2.0-2.3 A resolution, molecular replacement | Xanthomonas oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, complete inhibition | Xanthomonas oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Xanthomonas oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 D-alanine | Xanthomonas oryzae | - |
ADP + phosphate + D-alanyl-D-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas oryzae | Q5H614 | pv. oryzae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 D-alanine | - |
Xanthomonas oryzae | ADP + phosphate + D-alanyl-D-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ddl | - |
Xanthomonas oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Xanthomonas oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Xanthomonas oryzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Xanthomonas oryzae |
General Information | Comment | Organism |
---|---|---|
additional information | catalytic role of mega-loop, which contains two residues, K259 and Y260, which are crucial in substrate binding | Xanthomonas oryzae |