Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
ATCC 43589 | - |
Thermotoga maritima | P46805 | strain ATCC 43589 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.071 | - |
substrate beta-alanine, 60°C | Thermotoga maritima |
0.12 | - |
substrate D-threonine, 60°C | Thermotoga maritima |
0.2 | - |
substrate glycine, 60°C | Thermotoga maritima |
0.88 | - |
substrate D-serine, 60°C | Thermotoga maritima |
1.6 | - |
substrate D-cysteine, 60°C | Thermotoga maritima |
5.5 | - |
substrate D-alanine, 60°C | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-Ala | at 60°C activity is 1.3% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + ? | - |
? | |
ATP + D-alanine | - |
Thermotoga maritima | ADP + phosphate + D-alanyl-D-alanine | - |
? | |
ATP + D-Arg | at 60°C activity is 0.43% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Arg-D-Arg | - |
? | |
ATP + D-Asn | at 60°C activity is 0.22% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Asn-D-Asn | - |
? | |
ATP + D-Asp | at 60°C activity is 0.051% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Asp-D-Asp | - |
? | |
ATP + D-Cys | at 60°C activity is 29% of the activity with D-Ala | Thermotoga maritima | ADP + phosphate + D-Cys-D-Cys | - |
? | |
ATP + D-Gln | at 60°C activity is 0.56% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Gln-D-Gln | - |
? | |
ATP + D-Glu | at 60°C activity is 0.012% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Glu-D-Glu | - |
? | |
ATP + D-His | at 60°C activity is 0.49% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-His-D-His | - |
? | |
ATP + D-Ile | at 60°C activity is 0.36% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Ile-D-Ile | - |
? | |
ATP + D-Leu | at 60°C activity is 0.31% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Leu-D-Leu | - |
? | |
ATP + D-Lys | at 60°C activity is 0.6% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Lys-D-Lys | - |
? | |
ATP + D-Met | at 60°C activity is 0.34% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Met-D-Met | - |
? | |
ATP + D-Phe | at 60°C activity is 0.31% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Phe-D-Phe | - |
? | |
ATP + D-Pro | at 60°C activity is 0.29% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Pro-D-Pro | - |
? | |
ATP + D-Ser | at 60°C activity is 16% of the activity with D-Ala | Thermotoga maritima | ADP + phosphate + D-Ser-D-Ser | - |
? | |
ATP + D-Thr | at 60°C activity is 2.2% of the activity with D-Ala | Thermotoga maritima | ADP + phosphate + D-Thr-D-Thr | - |
? | |
ATP + D-Trp | at 60°C activity is 0.1% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Trp-D-Trp | - |
? | |
ATP + D-Val | at 60°C activity is 0.43% of the activity with D-Ala, no activity at 37°C | Thermotoga maritima | ADP + phosphate + D-Val-D-Val | - |
? | |
ATP + Gly | at 60°C activity is 3.6% of the activity with D-Ala | Thermotoga maritima | ADP + phosphate + Gly-Gly | - |
? | |
beta-alanine + beta-alanine + ATP | - |
Thermotoga maritima | beta-alanyl-beta-alanine + ADP + phosphate | 1.3% of the activity with D-serine | ? | |
D-alanine + D-alanine + ATP | - |
Thermotoga maritima | D-alanyl-D-alanine + ADP + phosphate | - |
? | |
D-cysteine + D-cysteine + ATP | - |
Thermotoga maritima | D-cysteinyl-D-cysteine + ADP + phosphate | 29% of the activity with D-serine | ? | |
D-serine + D-serine + ATP | - |
Thermotoga maritima | D-seryl-D-serine + ADP + phosphate | 16% of the activity with D-serine | ? | |
D-threonine + D-threonine + ATP | - |
Thermotoga maritima | D-threonyl-D-threonine + ADP + phosphate | 2.2% of the activity with D-serine | ? | |
glycine + D-alanine + ATP | - |
Thermotoga maritima | glycyl-D-alanine + ADP + phosphate | 3.6% of the activity with D-serine | ? | |
additional information | substrate specificity expands at high temperature. At 37°C, enzyme shows reactivity only for D-alanine, D-serine, D-threonine, D-cysteine and glycine. At 60°C, substrates additionally include D-lysine, D-glutamine, D-histidine, D-arginine, D-valine, D-isoleucine, D-methionine, D-leucine, D-phenylalanine, D-proline, and D-asparagine, all below 1% of the activity with D-alanine | Thermotoga maritima | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ddl | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: about 70% of maximal activity, 70°C: about 50% of maximal activity | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 90 | - |
Thermotoga maritima |
90 | - |
15 min, stable up to | Thermotoga maritima |