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Literature summary for 6.3.2.4 extracted from
- D-Alanyl-D-lactate and D-alanyl-D-alanine synthesis by D-alanyl-D-alanine ligase from vancomycin-resistant Leuconostoc mesenteroides. Effects of a phenylalanine 261 to tyrosine mutation (1997), J. Biol. Chem., 272, 9210-9214.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduction in Escherichia coli, the vancomycin resistant enzyme has Phe261 instead of Tyr. The Tyr to Phe substitution on the active site omega-loop in the D-Ala-D-Ala ligases is a molecular indicator of both the ability to make D-Ala-D-Lac and intrinsic resistance to the vancomycin class of glycopeptide antibiotics | Leuconostoc mesenteroides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Leuconostoc mesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | P71454 | resistant to vancomycin | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-Ala + D-Ala | - |
Leuconostoc mesenteroides | ADP + phosphate + D-Ala-D-Ala | - |
? |  |
| ATP + D-Ala + D-lactate | - |
Leuconostoc mesenteroides | ADP + phosphate + D-Ala-D-lactate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Leuconostoc mesenteroides |
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