BRENDA - Enzyme Database show
show all sequences of 6.3.2.36

Identification of pantoate kinase and phosphopantothenate synthetase from Methanospirillum hungatei

Katoh, H.; Tamaki, H.; Tokutake, Y.; Hanada, S.; Chohnan, S.; J. Biosci. Bioeng. 115, 372-376 (2013)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
acetyl-CoA
activates enzyme activity 1.64fold
Methanospirillum hungatei
CoA
activates enzyme activity 3.12fold
Methanospirillum hungatei
malonyl-CoA
activates enzyme activity 1.36fold
Methanospirillum hungatei
Cloned(Commentary)
Commentary
Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL, introduced with pSC101-based plasmid encoding argU, ileY, and leuW tRNA genes, subcloning in Escherichia coli strain JM109
Methanospirillum hungatei
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Methanospirillum hungatei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (R)-4-phosphopantoate + beta-alanine
Methanospirillum hungatei
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
ATP + (R)-4-phosphopantoate + beta-alanine
Methanospirillum hungatei JF-1
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanospirillum hungatei
Q2FUA9
-
-
Methanospirillum hungatei JF-1
Q2FUA9
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3) by nickel affinity chromatography, dialysis and ultrafiltration
Methanospirillum hungatei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-4-phosphopantoate + beta-alanine
-
722842
Methanospirillum hungatei
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
ATP + (R)-4-phosphopantoate + beta-alanine
-
722842
Methanospirillum hungatei JF-1
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Methanospirillum hungatei
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Methanospirillum hungatei
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Methanospirillum hungatei
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
acetyl-CoA
activates enzyme activity 1.64fold
Methanospirillum hungatei
CoA
activates enzyme activity 3.12fold
Methanospirillum hungatei
malonyl-CoA
activates enzyme activity 1.36fold
Methanospirillum hungatei
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL, introduced with pSC101-based plasmid encoding argU, ileY, and leuW tRNA genes, subcloning in Escherichia coli strain JM109
Methanospirillum hungatei
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Methanospirillum hungatei
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Methanospirillum hungatei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (R)-4-phosphopantoate + beta-alanine
Methanospirillum hungatei
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
ATP + (R)-4-phosphopantoate + beta-alanine
Methanospirillum hungatei JF-1
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3) by nickel affinity chromatography, dialysis and ultrafiltration
Methanospirillum hungatei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-4-phosphopantoate + beta-alanine
-
722842
Methanospirillum hungatei
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
ATP + (R)-4-phosphopantoate + beta-alanine
-
722842
Methanospirillum hungatei JF-1
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Methanospirillum hungatei
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Methanospirillum hungatei
Other publictions for EC 6.3.2.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746403
Kishimoto
Crystal structure of phosphop ...
Thermococcus kodakarensis, Thermococcus kodakarensis ATCC BAA-918 / JCM 12380 / KOD1
Proteins
82
1924-1936
2014
-
-
1
1
-
-
-
-
-
1
1
2
-
2
-
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
3
3
-
-
-
722842
Katoh
Identification of pantoate kin ...
Methanospirillum hungatei, Methanospirillum hungatei JF-1
J. Biosci. Bioeng.
115
372-376
2013
3
-
1
-
-
-
-
-
-
1
-
2
-
8
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
3
-
1
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
704547
Yokooji
Pantoate kinase and phosphopan ...
Thermococcus kodakarensis
J. Biol. Chem.
284
28137-28145
2009
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-