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Literature summary for 6.3.2.36 extracted from
- Identification of pantoate kinase and phosphopantothenate synthetase from Methanospirillum hungatei (2013), J. Biosci. Bioeng., 115, 372-376.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | activates enzyme activity 1.64fold | Methanospirillum hungatei |  |
| CoA | activates enzyme activity 3.12fold | Methanospirillum hungatei | |
| malonyl-CoA | activates enzyme activity 1.36fold | Methanospirillum hungatei | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL, introduced with pSC101-based plasmid encoding argU, ileY, and leuW tRNA genes, subcloning in Escherichia coli strain JM109 | Methanospirillum hungatei |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Methanospirillum hungatei | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-4-phosphopantoate + beta-alanine | Methanospirillum hungatei | - |
AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
| ATP + (R)-4-phosphopantoate + beta-alanine | Methanospirillum hungatei JF-1 | - |
AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanospirillum hungatei | Q2FUA9 | - |
- |
| Methanospirillum hungatei JF-1 | Q2FUA9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3) by nickel affinity chromatography, dialysis and ultrafiltration | Methanospirillum hungatei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-4-phosphopantoate + beta-alanine | - |
Methanospirillum hungatei | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
| ATP + (R)-4-phosphopantoate + beta-alanine | - |
Methanospirillum hungatei JF-1 | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mhun_0832 | - |
Methanospirillum hungatei |
| phosphopantothenate synthetase | - |
Methanospirillum hungatei |
| Pps | - |
Methanospirillum hungatei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Methanospirillum hungatei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Methanospirillum hungatei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Methanospirillum hungatei | |
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