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Literature summary for 6.3.2.35 extracted from

  • Weber, P.; Meziane-Cherif, D.; Haouz, A.; Saul, F.A.; Courvalin, P.
    Crystallization and preliminary X-ray analysis of a D-Ala:D-Ser ligase associated with VanG-type vancomycin resistance (2009), Acta Crystallogr. Sect. F, 65, 1024-1026.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.35

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecalis

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.35 A resolution, P3121 or P3221, with unit-cell parameters a and b 116.1, c 177.2 A Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39932
-
 x * 39947, MALDI-TOF, x * 39932, calculated for His-tagged protein Enterococcus faecalis
39947
-
 x * 39947, MALDI-TOF, x * 39932, calculated for His-tagged protein Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Enterococcus faecalis BM4518
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.2
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + D-serine + ATP
-
 Enterococcus faecalis D-alanyl-D-serine + ADP + phosphate
-
 ?
D-alanine + D-serine + ATP
-
 Enterococcus faecalis BM4518 D-alanyl-D-serine + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 39947, MALDI-TOF, x * 39932, calculated for His-tagged protein Enterococcus faecalis