Data extracted from this reference:
Cloned(Commentary)
expression in Escherichia coli
Methanocaldococcus jannaschii
Metals/Ions
Mg2+
conserved Mg-ATP-binding domain
Methanocaldococcus jannaschii
additional information
does not require K+ for activity
Methanocaldococcus jannaschii
Molecular Weight [Da]
33278
2 * 33278, calculated from sequence
Methanocaldococcus jannaschii
35000
2 * 35000, SDS-PAGE
Methanocaldococcus jannaschii
72000
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
ATP + tetrahydromethanopterin + L-glutamate
Methanocaldococcus jannaschii
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
GTP + tetrahydromethanopterin + L-glutamate
Methanocaldococcus jannaschii
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
Organism
Methanocaldococcus jannaschii
Q58037
Purification (Commentary)
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg]
12
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of ATP and K+
Methanocaldococcus jannaschii
12.7
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of GTP and K+
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
ATP + tetrahydromethanopterin + L-glutamate
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
ATP + tetrahydromethanopterin + L-glutamate
the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
GTP + tetrahydromethanopterin + L-glutamate
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
GTP + tetrahydromethanopterin + L-glutamate
the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
Subunits
homodimer
2 * 33278, calculated from sequence; 2 * 35000, SDS-PAGE
Methanocaldococcus jannaschii
Synonyms
H4MPT:alpha-L-glutamate ligase
Methanocaldococcus jannaschii
MJ0620
Methanocaldococcus jannaschii
MptN protein
Methanocaldococcus jannaschii
tetrahydromethanopterin:alpha-L-glutamate ligase
Methanocaldococcus jannaschii
Temperature Optimum [°C]
60
assay at
Methanocaldococcus jannaschii
pH Optimum
7
assay at
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
expression in Escherichia coli
Methanocaldococcus jannaschii
Metals/Ions (protein specific)
Mg2+
conserved Mg-ATP-binding domain
Methanocaldococcus jannaschii
additional information
does not require K+ for activity
Methanocaldococcus jannaschii
Molecular Weight [Da] (protein specific)
33278
2 * 33278, calculated from sequence
Methanocaldococcus jannaschii
35000
2 * 35000, SDS-PAGE
Methanocaldococcus jannaschii
72000
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
ATP + tetrahydromethanopterin + L-glutamate
Methanocaldococcus jannaschii
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
GTP + tetrahydromethanopterin + L-glutamate
Methanocaldococcus jannaschii
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
Purification (Commentary) (protein specific)
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg] (protein specific)
12
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of ATP and K+
Methanocaldococcus jannaschii
12.7
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of GTP and K+
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
ATP + tetrahydromethanopterin + L-glutamate
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
ATP + tetrahydromethanopterin + L-glutamate
the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
GTP + tetrahydromethanopterin + L-glutamate
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
GTP + tetrahydromethanopterin + L-glutamate
the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
?
Subunits (protein specific)
homodimer
2 * 33278, calculated from sequence; 2 * 35000, SDS-PAGE
Methanocaldococcus jannaschii
Temperature Optimum [°C] (protein specific)
60
assay at
Methanocaldococcus jannaschii
pH Optimum (protein specific)
7
assay at
Methanocaldococcus jannaschii
Other publictions for EC
700925
Li
Glutathione synthetase homolog ...
Methanocaldococcus jannaschii
Proc. Natl. Acad. Sci. USA
100
9785-9790
2003
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