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Literature summary for 6.3.2.16 extracted from

  • Reusch, V.M.; Neuhaus, F.C.
    D-Alanine:membrane acceptor ligase from Lactobacillus casei (1971), J. Biol. Chem., 246, 6136-6143.
    View publication on PubMed
Search for more literature for 6.3.2.16

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Lacticaseibacillus casei
D-2-amino-n-butanoate
-
 Lacticaseibacillus casei
D-Ser
-
 Lacticaseibacillus casei
DL-2-Amino-n-butanoyl hydroxamate
-
 Lacticaseibacillus casei
DL-Ala amide
-
 Lacticaseibacillus casei
DL-Alanine hydroxamate
-
 Lacticaseibacillus casei
Ni2+
-
 Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 D-Ala
-
 Lacticaseibacillus casei
0.85
-
 D-2-amino-n-butanoate
-
 Lacticaseibacillus casei
3.3
-
 ATP
-
 Lacticaseibacillus casei

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ stimulates Lacticaseibacillus casei
Ca2+ stimulates Lacticaseibacillus casei
Co2+ stimulates Lacticaseibacillus casei
Mg2+ stimulates Lacticaseibacillus casei
Mn2+ stimulates Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 gel filtration Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-Ala + membrane-acceptor Lacticaseibacillus casei reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + D-Ala + membrane-acceptor 83% of the activity relative to ATP Lacticaseibacillus casei AMP + phosphate + D-alanyl-membrane acceptor
-
 ?
ATP + D-2-amino-n-butanoate + membrane-acceptor
-
 Lacticaseibacillus casei ADP + phosphate + D-2-aminobutanoyl-membrane acceptor
-
 ?
ATP + D-Ala + membrane-acceptor no activity with L-Ala Lacticaseibacillus casei ADP + phosphate + D-alanyl-membrane acceptor
-
 ?
ATP + D-Ala + membrane-acceptor reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids Lacticaseibacillus casei ?
-
 ?
CTP + D-Ala + membrane-acceptor 107% of the activity relative to ATP Lacticaseibacillus casei CDP + phosphate + D-alanyl-membrane acceptor
-
 ?
GTP + D-Ala + membrane-acceptor 84% of the activity relative to ATP Lacticaseibacillus casei GDP + phosphate + D-alanyl-membrane acceptor
-
 ?
UTP + D-Ala + membrane-membrane acceptor 126% of the activity relative to ATP Lacticaseibacillus casei UDP + phosphate + D-alanyl-membrane acceptor
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Lacticaseibacillus casei