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Literature summary for 6.3.1.8 extracted from

  • Sousa, A.F.; Gomes-Alves, A.G.; Benitez, D.; Comini, M.A.; Flohe, L.; Jaeger, T.; Passos, J.; Stuhlmann, F.; Tomas, A.M.; Castro, H.
    Genetic and chemical analyses reveal that trypanothione synthetase but not glutathionylspermidine synthetase is essential for Leishmania infantum (2014), Free Radic. Biol. Med., 73, 229-238 .
    View publication on PubMed
Search for more literature for 6.3.1.8

Application

Application Comment Organism
additional information GSPS is no valuable target for antiparasitic drug development Leishmania infantum

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) in inclusion bodies Leishmania infantum

Protein Variants

Protein Variants Comment Organism
additional information generation of a knockout GSPS mutant line by gene targeting, the mutant is viable and capable of replicating in both life cycle stages of the parasite, the extracellular promastigotes residing in the insect vector and the intracellular amastigotes thriving in mammalian hosts Leishmania infantum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Leishmania infantum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + spermidine + ATP Leishmania infantum
-
 glutathionylspermidine + ADP + phosphate
-
 ?
glutathione + spermidine + ATP Leishmania infantum MHOM / MA / 67 / ITMAP263
-
 glutathionylspermidine + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania infantum A4I1T8
-
-
Leishmania infantum MHOM / MA / 67 / ITMAP263 A4I1T8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme solubilized from Escherichia coli strain Rosetta (DE3) inclusion bodies by nickel affinity chromatography and gel filtration Leishmania infantum

Renatured (Commentary)

Renatured (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) inclusion bodies by solubilization with 8 M urea, 5 mM imidazole, and 1 mM 2-mercaptoethanol Leishmania infantum

Source Tissue

Source Tissue Comment Organism Textmining
additional information the extracellular promastigotes residing in the insect vector and the intracellular amastigotes thriving in mammalian hosts, expression of LiGSPS cannot be detected directly in Leishmania infantum Leishmania infantum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + spermidine + ATP
-
 Leishmania infantum glutathionylspermidine + ADP + phosphate
-
 ?
glutathione + spermidine + ATP
-
 Leishmania infantum MHOM / MA / 67 / ITMAP263 glutathionylspermidine + ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GspS
-
 Leishmania infantum
LiGSPS
-
 Leishmania infantum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Leishmania infantum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Leishmania infantum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Leishmania infantum

General Information

General Information Comment Organism
physiological function glutathionylspermidine synthetase (GSPS) is capable of performing the first step of trypanothione synthesis yielding glutathionylspermidine, this monothiol can substitute for trypanothione in some reactions. But trypanothione synthetase, EC 6.3.1.9, and not glutathionylspermidine synthetase, EC 6.3.1.8, is essential for Leishmania infantum, superfluous role of enzyme GSPS. LiGSPS is not crucial for survival and replication of Leishmania infantum promastigotes and amastigotes, trypanothione synthase, TRYS, might be redundant in parasites harboring GSPS Leishmania infantum