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Literature summary for 6.3.1.8 extracted from

  • Comini, M.A.; Guerrero, S.A.; Haile, S.; Menge, U.; Lunsdorf, H.; Flohe, L.
    Valdiation of Trypanosoma brucei trypanothione synthetase as drug target (2004), Free Radic. Biol. Med., 36, 1289-1302.
    View publication on PubMed
Search for more literature for 6.3.1.8

Application

Application Comment Organism
drug development the enzyme is a target for drug development Trypanosoma brucei

Cloned(Commentary)

Cloned (Comment) Organism
enzyme expression analysis, cloning and transfection of RNAi Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
additional information enzyme downregulation leads to increased trypanocidic drug sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine and glutathione accumulation, RNA interference method, overview Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
buthionine sulfoximine
-
 Trypanosoma brucei
megazol
-
 Trypanosoma brucei
Melarsen oxide
-
 Trypanosoma brucei
additional information the enzyme mediates resistance to H2O2 and organic hyperoxids, and to trypanocidic drugs Trypanosoma brucei
nifurtimox
-
 Trypanosoma brucei
Pentamidine
-
 Trypanosoma brucei
triostam
-
 Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + spermidine + ATP Trypanosoma brucei trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?
additional information Trypanosoma brucei the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
 bifunctional enzyme with glutathionylspermidine synthase and trypanothione synthase activity
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?
additional information the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction Trypanosoma brucei ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Trypanosoma brucei