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Literature summary for 6.3.1.20 extracted from
- The Thermoplasma acidophilum LplA-LplB complex defines a new class of bipartite lipoate-protein ligases (2009), J. Biol. Chem., 284, 21317-21326.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thermoplasma acidophilum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11200 | - |
1 * 30800 + 1 * 11200, LplA-LplB complex, gel filtration | Thermoplasma acidophilum |
| 30800 | - |
1 * 30800 + 1 * 11200, LplA-LplB complex, gel filtration | Thermoplasma acidophilum |
| 41100 | - |
LplA-LplB complex, gel filtration | Thermoplasma acidophilum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Thermoplasma acidophilum | LplA has no detectable ligase activity in vitro in the absence of LplB | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma acidophilum | Q9HKT1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA-agarose resin column chromatography and POROS HQ 20 column chromatography | Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-lipoic acid | - |
Thermoplasma acidophilum | diphosphate + D-lipoyl-AMP | - |
? | |
| additional information | LplA has no detectable ligase activity in vitro in the absence of LplB | Thermoplasma acidophilum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 30800 + 1 * 11200, LplA-LplB complex, gel filtration | Thermoplasma acidophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipoate ligase | - |
Thermoplasma acidophilum |
| lipoyl ligase | - |
Thermoplasma acidophilum |
| LPLA | - |
Thermoplasma acidophilum |
| LplA-LplB complex | - |
Thermoplasma acidophilum |
| LplB | - |
Thermoplasma acidophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Thermoplasma acidophilum | |
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Release 2023.1 (January 2023)
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