Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis ATCC 25618 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme expressed in Escherichia coli | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
PafA | ambiguous | Mycobacterium tuberculosis |
Pup ligase | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | proteasome-containing bacteria possess a tagging system that directs proteins to proteasomal degradation by conjugating them to a prokaryotic ubiquitin-like protein (Pup). A single ligating enzyme, PafA, is responsible for Pup conjugation to lysine side chains of protein substrates. As Pup is recognized by the regulatory subunit of the proteasome, Pup functions as a degradation tag. Ligating enzyme PafA and the proteasome can function as a modular machine for the tagging and degradation of cytoplasmic proteins | Mycobacterium tuberculosis |