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Literature summary for 6.2.1.71 extracted from
- Vibriobactin biosynthesis in Vibrio cholerae VibH is an amide synthase homologous to nonribosomal peptide synthetase condensation domains (2000), Biochemistry, 39, 15513-15521 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vibrio cholerae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00046 | - |
2,3-Dihydroxybenzoate | pH 7.5, 30°C | Vibrio cholerae |  |
| 0.016 | - |
salicylate | pH 7.5, 30°C | Vibrio cholerae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae | Q9FDB2 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2,3-dihydroxybenzoate + [aryl-carrier protein EntB] | - |
Vibrio cholerae | AMP + diphosphate + 2,3-dihydroxybenzoyl-[aryl-carrier protein EntB] | - |
? | |
| ATP + salicylate + holo-[aryl-carrier protein EntB] | - |
Vibrio cholerae | AMP + diphosphate + salicyl-[aryl-carrier protein EntB] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2,3-dihydroxybenzoate-AMP ligase | - |
Vibrio cholerae |
| vibE | - |
Vibrio cholerae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
salicylate | pH 7.5, 30°C | Vibrio cholerae | |
| 2.3 | - |
2,3-Dihydroxybenzoate | pH 7.5, 30°C | Vibrio cholerae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | during biosynthesis of Vibrio cholerae siderophore vibriobactin, VibE activates 2,3-dihydroxybenzoate as the acyl adenylate and then transfers it to the free thiol of the phosphopantetheine arm of VibB's aryl carrier domain | Vibrio cholerae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
salicylate | pH 7.5, 30°C | Vibrio cholerae | |
| 5000 | - |
2,3-Dihydroxybenzoate | pH 7.5, 30°C | Vibrio cholerae | |
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Release 2023.1 (January 2023)
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