Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
A189T | improved stability and activity compared to mutant A189T/W714C, but when incubated at 39°C, cells expressing the mutant show increased apoptotic rate ompared to wild-type. Mutant is able to monoubiquitinate histone H2A and to support growth of TS20 cells at 39°C. Compared to mutant A189T/W714C, mutation A189T significantly improves the ubiquitination-dependent disposal of HIF-1alpha | Mus musculus |
A189T/W714C | mutant protein is less stable than its wildtype counterpart, and restrictive temperature of 39°C accelerates its degradation | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A31N-ts20 cell | mouse embryo fibroblast cell, thermosensitive for ubiquitin-activating enzyme E1 | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ubiquitin + [ubiquitin-activating protein E1]-L-cysteine | - |
Mus musculus | AMP + diphosphate + [ubiquitin-activating protein E1]-S-ubiquitinyl-L-cysteine | - |
? |