Any feedback?
Literature summary for 6.2.1.43 extracted from
- Characterization of NcsB2 as a promiscuous naphthoic acid/coenzyme A ligase integral to the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin (2007), J. Am. Chem. Soc., 129, 7728-7729.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Streptomyces carzinostaticus |
| expression in Escherichia coli | Streptomyces carzinostaticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
2-hydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus |  |
| 0.00025 | - |
2-hydroxy-5-methylnaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.00059 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.00059 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.0011 | - |
2-hydroxy-5,7-dimethoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.0011 | - |
2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.0023 | - |
2-hydroxy-7-methoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.0023 | - |
2-hydroxy-7-methoxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.0031 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.0031 | - |
2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.0034 | - |
2-hydroxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.0034 | - |
2-hydroxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.018 | - |
1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.018 | - |
Naphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | Streptomyces carzinostaticus | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | Streptomyces carzinostaticus | 100% activity | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | Streptomyces carzinostaticus ATCC 15944 | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces carzinostaticus | - |
- |
- |
| Streptomyces carzinostaticus ATCC 15944 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces carzinostaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-naphthoate + CoA | kcat/Km is 0.6% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 1-naphthoyl-CoA | - |
? | |
| ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA | kcat/Km is 47% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA | kcat/Km is 47% compared to the wild-type value | Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid + CoA | 47% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
| ATP + 2-hydroxy-1-naphthoate + CoA | kcat/Km is 1.3% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-5,7-dimethoxy-1-naphthoate + CoA | kcat/Km is 35% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-5,7-dimethoxy-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid + CoA | 35% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
| ATP + 2-hydroxy-5-methyl-1-naphthoate + CoA | kcat/Km is 50% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-5-methyl-1-naphthoate + CoA | kcat/Km is 50% compared to the wild-type value | Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2-hydroxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-5-methylnaphthalene-1-carboxylic acid + CoA | 50% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
| ATP + 2-hydroxy-7-methoxy-1-naphthoate + CoA | kcat/Km is 5% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-7-methoxy-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-7-methoxy-1-naphthoate + CoA | kcat/Km is 5% compared to the wild-type value | Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2-hydroxy-7-methoxy-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | - |
Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | 100% activity | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | - |
Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
| ATP + 2-hydroxy-7-methoxynaphthalene-1-carboxylic acid + CoA | 4.8% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
| ATP + 2-hydroxynaphthalene-1-carboxylic acid + CoA | 1.3% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
| ATP + naphthalene-1-carboxylic acid + CoA | 0.58% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| naphthoic acid/coenzyme A ligase | - |
Streptomyces carzinostaticus |
| naphthoyl CoA ligase | - |
Streptomyces carzinostaticus |
| NcsB2 | - |
Streptomyces carzinostaticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.047 | - |
2-hydroxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.05 | - |
2-hydroxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.11 | - |
1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.11 | - |
Naphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.118 | - |
2-hydroxy-7-methoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.12 | - |
2-hydroxy-7-methoxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.135 | - |
2-hydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.135 | - |
2-hydroxy-5-methylnaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.42 | - |
2-hydroxy-5,7-dimethoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.42 | - |
2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 0.63 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 0.63 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
| 1.55 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 1.55 | - |
2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.8 | - |
2-hydroxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 51.3 | - |
2-hydroxy-7-methoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 382 | - |
2-hydroxy-5,7-dimethoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 500 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 540 | - |
2-hydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
| 1068 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
