BRENDA - Enzyme Database
show all sequences of 6.2.1.43

Characterization of NcsB2 as a promiscuous naphthoic acid/coenzyme A ligase integral to the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin

Cooke, H.A.; Zhang, J.; Griffin, M.A.; Nonaka, K.; van Lanen, S.G.; Shen, B.; Bruner, S.D.; J. Am. Chem. Soc. 129, 7728-7729 (2007) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli
Streptomyces carzinostaticus
expression in Escherichia coli
Streptomyces carzinostaticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00025
-
2-hydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.00025
-
2-hydroxy-5-methylnaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.00059
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.00059
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.0011
-
2-hydroxy-5,7-dimethoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.0011
-
2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.0023
-
2-hydroxy-7-methoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.0023
-
2-hydroxy-7-methoxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.0031
-
2,7-dihydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.0031
-
2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.0034
-
2-hydroxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.0034
-
2-hydroxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.018
-
1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.018
-
Naphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
Streptomyces carzinostaticus
100% activity
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
Streptomyces carzinostaticus
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
Streptomyces carzinostaticus ATCC 15944
100% activity
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
Streptomyces carzinostaticus ATCC 15944
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces carzinostaticus
-
-
-
Streptomyces carzinostaticus ATCC 15944
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Streptomyces carzinostaticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + 1-naphthoate + CoA
kcat/Km is 0.6% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 1-naphthoyl-CoA
-
-
-
?
ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA
kcat/Km is 47% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA
kcat/Km is 47% compared to the wild-type value
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid + CoA
47% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + 2-hydroxy-1-naphthoate + CoA
kcat/Km is 1.3% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-1-naphthoate + CoA
kcat/Km is 1.3% compared to the wild-type value
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2-hydroxy-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-5,7-dimethoxy-1-naphthoate + CoA
kcat/Km is 35% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-5,7-dimethoxy-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid + CoA
35% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + 2-hydroxy-5-methyl-1-naphthoate + CoA
kcat/Km is 50% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-5-methylnaphthalene-1-carboxylic acid + CoA
50% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + 2-hydroxy-7-methoxy-1-naphthoate + CoA
kcat/Km is 5% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-7-methoxy-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
-
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
100% activity
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
-
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
100% activity
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxynaphthalene-1-carboxylic acid + CoA
4.8% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + 2-hydroxynaphthalene-1-carboxylic acid + CoA
1.3% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + naphthalene-1-carboxylic acid + CoA
0.58% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
naphthoic acid/coenzyme A ligase
-
Streptomyces carzinostaticus
naphthoyl CoA ligase
-
Streptomyces carzinostaticus
NcsB2
-
Streptomyces carzinostaticus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.047
-
2-hydroxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.05
-
2-hydroxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.11
-
Naphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.11
-
1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.118
-
2-hydroxy-7-methoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.12
-
2-hydroxy-7-methoxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.135
-
2-hydroxy-5-methylnaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.135
-
2-hydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.42
-
2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.42
-
2-hydroxy-5,7-dimethoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.63
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.63
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
1.55
-
2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
1.55
-
2,7-dihydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Streptomyces carzinostaticus
expression in Escherichia coli
Streptomyces carzinostaticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00025
-
2-hydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.00025
-
2-hydroxy-5-methylnaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.00059
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.00059
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.0011
-
2-hydroxy-5,7-dimethoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.0011
-
2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.0023
-
2-hydroxy-7-methoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.0023
-
2-hydroxy-7-methoxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.0031
-
2,7-dihydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.0031
-
2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.0034
-
2-hydroxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.0034
-
2-hydroxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.018
-
1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.018
-
Naphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
Streptomyces carzinostaticus
100% activity
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
Streptomyces carzinostaticus
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
Streptomyces carzinostaticus ATCC 15944
100% activity
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
Streptomyces carzinostaticus ATCC 15944
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces carzinostaticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + 1-naphthoate + CoA
kcat/Km is 0.6% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 1-naphthoyl-CoA
-
-
-
?
ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA
kcat/Km is 47% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA
kcat/Km is 47% compared to the wild-type value
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid + CoA
47% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + 2-hydroxy-1-naphthoate + CoA
kcat/Km is 1.3% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-1-naphthoate + CoA
kcat/Km is 1.3% compared to the wild-type value
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2-hydroxy-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-5,7-dimethoxy-1-naphthoate + CoA
kcat/Km is 35% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-5,7-dimethoxy-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid + CoA
35% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + 2-hydroxy-5-methyl-1-naphthoate + CoA
kcat/Km is 50% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-5-methylnaphthalene-1-carboxylic acid + CoA
50% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + 2-hydroxy-7-methoxy-1-naphthoate + CoA
kcat/Km is 5% compared to the wild-type value
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-7-methoxy-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
-
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
100% activity
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
725189
Streptomyces carzinostaticus
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
-
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
100% activity
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
725189
Streptomyces carzinostaticus ATCC 15944
AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
-
-
?
ATP + 2-hydroxy-7-methoxynaphthalene-1-carboxylic acid + CoA
4.8% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + 2-hydroxynaphthalene-1-carboxylic acid + CoA
1.3% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
ATP + naphthalene-1-carboxylic acid + CoA
0.58% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate
725189
Streptomyces carzinostaticus
AMP + diphosphate + ?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.047
-
2-hydroxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.05
-
2-hydroxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.11
-
Naphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.11
-
1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.118
-
2-hydroxy-7-methoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.12
-
2-hydroxy-7-methoxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.135
-
2-hydroxy-5-methylnaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.135
-
2-hydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.42
-
2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.42
-
2-hydroxy-5,7-dimethoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
0.63
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH and temperature not specified in the publication
Streptomyces carzinostaticus
0.63
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
1.55
-
2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid
pH and temperature not specified in the publication
Streptomyces carzinostaticus
1.55
-
2,7-dihydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
Streptomyces carzinostaticus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin
Streptomyces carzinostaticus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
13.8
-
2-hydroxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
51.3
-
2-hydroxy-7-methoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
382
-
2-hydroxy-5,7-dimethoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
500
-
2,7-dihydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
540
-
2-hydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
1068
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
13.8
-
2-hydroxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
51.3
-
2-hydroxy-7-methoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
382
-
2-hydroxy-5,7-dimethoxy-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
500
-
2,7-dihydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
540
-
2-hydroxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
1068
-
2-hydroxy-7-methoxy-5-methyl-1-naphthoate
pH 8.0, 24°C
Streptomyces carzinostaticus
Other publictions for EC 6.2.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725189
Cooke
Characterization of NcsB2 as a ...
Streptomyces carzinostaticus, Streptomyces carzinostaticus ATCC 15944
J. Am. Chem. Soc.
129
7728-7729
2007
-
-
2
-
-
-
-
14
-
-
-
4
-
3
-
-
1
-
-
-
-
-
20
-
3
-
-
-
14
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
14
-
-
-
4
-
-
-
1
-
-
-
-
20
-
-
-
-
14
-
-
-
-
-
1
1
-
6
6