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Literature summary for 6.2.1.4 extracted from
- Structural basis for the binding of succinate to succinyl-CoA synthetase (2016), Acta Crystallogr. Sect. D, 72, 912-921 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Sus scrofa |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with succinate, magnesium ions and CoA, hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350 and 0.2 M magnesium formate | Sus scrofa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| V330M | the mutant exhibits an about 10fold higher Km value for succinate compared to the wild type enzyme | Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
succinate | wild type enzyme, at pH 7.4 and 21°C | Sus scrofa |  |
| 5.1 | - |
succinate | mutant enzyme V330M, at pH 7.4 and 21°C | Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Sus scrofa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + succinate + CoA | Sus scrofa | - |
GDP + phosphate + succinyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | P53590 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, HiTrap Blue column chromatography, and Sephadex G-25 gel filtration | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + L-malate + CoA | low activity | Sus scrofa | GDP + phosphate + L-malyl-CoA | - |
? | |
| GTP + succinate + CoA | - |
Sus scrofa | GDP + phosphate + succinyl-CoA | - |
? | |
| additional information | no activity with citrate, fumarate and oxaloacetate | Sus scrofa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GTP-specific succinyl-CoA synthetase | - |
Sus scrofa |
| GTPSCS | - |
Sus scrofa |
| SUCLG2 | - |
Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| GTP | - |
Sus scrofa | |
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Release 2023.1 (January 2023)
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