Application | Comment | Organism |
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synthesis | engineering of Hansenula polymorpha for secretion of penicillin. Introduction of the Penicilliumchrysogenum gene encoding the non-ribosomal peptide synthetase delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase in Hansenula polymorpha, results in the production of active enzyme, when co-expressed with the Bacillus subtilis sfp gene encoding a phosphopantetheinyl transferase that activates ACVS. Co-expression with the Penicillium chrysogenum genes encoding the cytosolic enzyme isopenicillin N synthase as well as the two peroxisomal enzymes isopenicillin N acyl transferase and phenylacetyl CoA ligase results in production of biologically active penicillin, which is efficiently secreted. The amount of secreted penicillin is approximately 1 mg/l. Penicillin production is decreased over 2fold in a yeast strain lacking peroxisomes | Penicillium chrysogenum |
Cloned (Comment) | Organism |
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- |
Penicillium chrysogenum |
Organism | UniProt | Comment | Textmining |
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Penicillium chrysogenum | - |
- |
- |