Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Dihydroxybenzoate | strong | Pseudomonas sp. | |
2-hydroxy-4-methylbenzoate | strong | Pseudomonas sp. | |
5,5'-dithiobis(2-nitrobenzoate) | - |
Pseudomonas sp. | |
PCMB | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
4-hydroxybenzoate | pH 7.8, 37°C | Pseudomonas sp. | |
0.077 | - |
ATP | pH 7.8, 37°C | Pseudomonas sp. | |
0.125 | - |
coenzyme A | pH 7.8, 37°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-hydroxybenzoate + CoA | Pseudomonas sp. | initial step of anaerobic 4-hydroxybenzoate degradation | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
ATP + 4-hydroxybenzoate + CoA | Pseudomonas sp. K172 | initial step of anaerobic 4-hydroxybenzoate degradation | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. K172 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen-sensitive during initial stages of purification, can be stabilized by adding DTT and 10% glycerol | Pseudomonas sp. |
Purification (Comment) | Organism |
---|---|
strain K172 | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:4-hydroxybenzoate-grown cell | in the mid-exponential growth phase | Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.3 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-aminobenzoate + CoA | 30% of the activity with 4-hydroxybenzoate | Pseudomonas sp. | AMP + 4-aminobenzoyl-CoA + diphosphate | - |
? | |
ATP + 4-fluorobenzoate + CoA | 3.8% of the activity with 4-hydroxybenzoate | Pseudomonas sp. | AMP + 4-fluorobenzoyl-CoA + diphosphate | - |
? | |
ATP + 4-hydroxybenzoate + CoA | highly specific | Pseudomonas sp. | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
ATP + 4-hydroxybenzoate + CoA | anaerob | Pseudomonas sp. | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
ATP + 4-hydroxybenzoate + CoA | initial step of anaerobic 4-hydroxybenzoate degradation | Pseudomonas sp. | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
ATP + 4-hydroxybenzoate + CoA | highly specific | Pseudomonas sp. K172 | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
ATP + 4-hydroxybenzoate + CoA | anaerob | Pseudomonas sp. K172 | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
ATP + 4-hydroxybenzoate + CoA | initial step of anaerobic 4-hydroxybenzoate degradation | Pseudomonas sp. K172 | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
ATP + phenylacetate + CoA | 4.5% of the activity with 4-hydroxybenzoate | Pseudomonas sp. | AMP + phenylacetyl-CoA + diphosphate | - |
? | |
CTP + 4-hydroxybenzoate + CoA | 60% of the activity with ATP | Pseudomonas sp. | CMP + 4-hydroxybenzoyl-CoA + diphosphate | - |
? | |
CTP + 4-hydroxybenzoate + CoA | 60% of the activity with ATP | Pseudomonas sp. K172 | CMP + 4-hydroxybenzoyl-CoA + diphosphate | - |
? | |
additional information | not: benzoate, 3- or 2-hydroxybenzoate | Pseudomonas sp. | ? | - |
? | |
additional information | not: benzoate, 3- or 2-hydroxybenzoate | Pseudomonas sp. K172 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48000, SDS-PAGE | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
4-hydroxybenzoate | pH 7.8, 37°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.5 | - |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | 50% of activity maximum at pH 7.0 and 9.5, almost no activity at pH 6.0 and 10.0 | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
2,4-Dihydroxybenzoate | pH 7.8, 37°C | Pseudomonas sp. | |
0.0014 | - |
2-hydroxy-4-methylbenzoate | pH 7.8, 37°C | Pseudomonas sp. |