Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoyl-CoA | product inhibition | Rhodopseudomonas palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | 0.002 | benzoate | - |
Rhodopseudomonas palustris | |
0.002 | 0.003 | ATP | - |
Rhodopseudomonas palustris | |
0.09 | 0.12 | reduced CoA | - |
Rhodopseudomonas palustris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rhodopseudomonas palustris | |
Mn2+ | can replace Mg2+ | Rhodopseudomonas palustris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
HPLC gel filtration | Rhodopseudomonas palustris |
60000 | - |
1 * 60000, SDS-PAGE | Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + benzoate + CoA | Rhodopseudomonas palustris | enzyme is involved in anaerobic degradation of benzoate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | - |
- |
- |
Oxidation Stability | Organism |
---|---|
purified enzyme is insensitive to oxygen | Rhodopseudomonas palustris |
Purification (Comment) | Organism |
---|---|
- |
Rhodopseudomonas palustris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Rhodopseudomonas palustris |
Storage Stability | Organism |
---|---|
0°C, 1 mg/ml purified enzyme, half-life is 8 days | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-chlorobenzoate + CoA | 10% of the activity relative to benzoate | Rhodopseudomonas palustris | AMP + diphosphate + 2-chlorobenzoyl-CoA | - |
? | |
ATP + 2-fluorobenzoate + CoA | 102% of the activity relative to benzoate | Rhodopseudomonas palustris | AMP + diphosphate + 2-fluorobenzoyl-CoA | - |
? | |
ATP + 4-fluorobenzoate + CoA | 72% of the activity relative to benzoate | Rhodopseudomonas palustris | AMP + diphosphate + 4-fluorobenzoyl-CoA | - |
? | |
ATP + benzoate + CoA | - |
Rhodopseudomonas palustris | AMP + diphosphate + benzoyl-CoA | - |
? | |
ATP + benzoate + CoA | enzyme is involved in anaerobic degradation of benzoate | Rhodopseudomonas palustris | ? | - |
? | |
ATP + DELTA1-cyclohexenecarboxylate + CoA | 13% of the activity relative to benzoate | Rhodopseudomonas palustris | AMP + diphosphate + DELTA1-cyclohexenecarboxyl-CoA | - |
? | |
ATP + DELTA3-cyclohexenecarboxylate + CoA | 54% of the activity relative to benzoate | Rhodopseudomonas palustris | AMP + diphosphate + DELTA3-cyclohexenecarboxyl-CoA | - |
? | |
ATP + nicotinate + CoA | 12% of the activity relative to benzoate | Rhodopseudomonas palustris | AMP + diphosphate + nicotinyl-CoA | - |
? | |
ATP + picolinate + CoA | 45% of the activity relative to benzoate | Rhodopseudomonas palustris | AMP + diphosphate + picolinyl-CoA | - |
? | |
additional information | no reaction with GTP | Rhodopseudomonas palustris | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Rhodopseudomonas palustris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.9 | - |
Rhodopseudomonas palustris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.9 | 7.0: 10% of maximal activity, 8.0: 75% of maximal activity, 8.4-8.9: maximal activity | Rhodopseudomonas palustris |