BRENDA - Enzyme Database show
show all sequences of 6.2.1.2

The macrophage-induced gene (mig) of Mycobacterium avium encodes a medium-chain acyl-coenzyme A synthetase

Morsczeck, C.; Berger, S.; Plum, G.; Biochim. Biophys. Acta 1521, 59-65 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
a plasmid with a His-tagged fusion protein is constructed for expression in Escherichia coli
Mycobacterium avium
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-hydroxydodecanoic acid
-
Mycobacterium avium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
dodecanoate
-
Mycobacterium avium
0.036
-
ATP
-
Mycobacterium avium
0.045
-
CoA
-
Mycobacterium avium
0.285
-
Octanoate
-
Mycobacterium avium
1.775
-
Decanoate
-
Mycobacterium avium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mycobacterium avium
the enzyme is involved in the metabolism of fatty acid during mycobacterial survival in macrophages
?
-
Mycobacterium avium
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium avium
-
-
-
Purification (Commentary)
Commentary
Organism
-
Mycobacterium avium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-aminobenzoate + CoA
1.4% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + 2-aminobenzoyl-CoA
-
650334
Mycobacterium avium
?
ATP + arachidonic acid + CoA
5.2% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + arachidonoyl-CoA
-
650334
Mycobacterium avium
?
ATP + benzoate + CoA
2.3% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + benzoyl-CoA
-
650334
Mycobacterium avium
?
ATP + decanoate + CoA
substrate with the highest activity
650334
Mycobacterium avium
AMP + diphosphate + decanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + dodecanoate + CoA
31% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + dodecanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + heptanoate + CoA
34% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + heptanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + hexanoate + CoA
35% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + hexanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + linolenic acid + CoA
8.3% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + linolenoyl-CoA
-
650334
Mycobacterium avium
?
ATP + m-hydroxybenzoate + CoA
0.8% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + m-hydroxybenzoyl-CoA
-
650334
Mycobacterium avium
?
ATP + octanoate + CoA
31% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + octanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + pentanoate + CoA
1.6% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + pentanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + tetradecanoate + CoA
3.9% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + tetradecanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + tridecanoate + CoA
15% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + tridecanoyl-CoA
-
650334
Mycobacterium avium
?
additional information
the enzyme is involved in the metabolism of fatty acid during mycobacterial survival in macrophages
650334
Mycobacterium avium
?
-
650334
Mycobacterium avium
?
Cloned(Commentary) (protein specific)
Commentary
Organism
a plasmid with a His-tagged fusion protein is constructed for expression in Escherichia coli
Mycobacterium avium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-hydroxydodecanoic acid
-
Mycobacterium avium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
dodecanoate
-
Mycobacterium avium
0.036
-
ATP
-
Mycobacterium avium
0.045
-
CoA
-
Mycobacterium avium
0.285
-
Octanoate
-
Mycobacterium avium
1.775
-
Decanoate
-
Mycobacterium avium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mycobacterium avium
the enzyme is involved in the metabolism of fatty acid during mycobacterial survival in macrophages
?
-
Mycobacterium avium
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mycobacterium avium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-aminobenzoate + CoA
1.4% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + 2-aminobenzoyl-CoA
-
650334
Mycobacterium avium
?
ATP + arachidonic acid + CoA
5.2% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + arachidonoyl-CoA
-
650334
Mycobacterium avium
?
ATP + benzoate + CoA
2.3% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + benzoyl-CoA
-
650334
Mycobacterium avium
?
ATP + decanoate + CoA
substrate with the highest activity
650334
Mycobacterium avium
AMP + diphosphate + decanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + dodecanoate + CoA
31% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + dodecanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + heptanoate + CoA
34% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + heptanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + hexanoate + CoA
35% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + hexanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + linolenic acid + CoA
8.3% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + linolenoyl-CoA
-
650334
Mycobacterium avium
?
ATP + m-hydroxybenzoate + CoA
0.8% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + m-hydroxybenzoyl-CoA
-
650334
Mycobacterium avium
?
ATP + octanoate + CoA
31% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + octanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + pentanoate + CoA
1.6% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + pentanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + tetradecanoate + CoA
3.9% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + tetradecanoyl-CoA
-
650334
Mycobacterium avium
?
ATP + tridecanoate + CoA
15% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + tridecanoyl-CoA
-
650334
Mycobacterium avium
?
additional information
the enzyme is involved in the metabolism of fatty acid during mycobacterial survival in macrophages
650334
Mycobacterium avium
?
-
650334
Mycobacterium avium
?
Other publictions for EC 6.2.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727750
Meng
Characterization of an archaea ...
Methanosarcina acetivorans, Methanosarcina acetivorans DSM 2834
J. Bacteriol.
192
5982-5990
2010
-
-
1
-
-
-
-
3
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
1
-
-
3
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
3
1
-
-
-
-
-
-
-
3
3
690742
Blacklock
A fatty acid elongase ELO with ...
Dictyostelium discoideum
Biochem. Biophys. Res. Commun.
374
226-230
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
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-
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-
-
-
-
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-
-
1
-
-
-
-
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690837
Koetsier
Characterization of a phenylac ...
Penicillium chrysogenum
Biochem. J.
417
467-476
2008
-
-
1
-
6
1
-
10
-
2
1
1
-
1
-
-
1
-
-
-
2
1
12
2
1
-
-
7
1
-
-
1
1
-
-
-
-
1
1
-
6
1
-
-
1
10
-
2
1
1
-
-
-
1
-
-
2
1
12
2
1
-
-
7
1
-
-
-
-
-
-
-
-
-
675838
Arabolaza
Transcriptional regulation of ...
Streptomyces coelicolor
Microbiology
152
1427-1439
2006
-
-
1
-
-
-
-
-
-
-
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-
1
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1
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-
676089
Lindner
The L513S polymorphism in medi ...
Homo sapiens
Mol. Nutr. Food Res.
50
270-274
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
676959
Kasuya
Purification, characterization ...
Bos taurus, Mus musculus
Protein Expr. Purif.
47
405-414
2006
-
-
-
-
-
-
10
-
5
-
1
-
-
5
-
-
2
-
-
5
2
-
28
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
10
2
-
5
-
1
-
-
-
-
2
-
5
2
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651834
Vessey
Isolation, sequencing, and exp ...
Homo sapiens
J. Biochem. Mol. Toxicol.
17
1-6
2003
-
-
1
-
-
-
-
-
2
-
-
-
-
4
-
-
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649709
Vessey
Characterization of the reacti ...
Bos taurus
Biochem. J.
357
283-288
2001
-
-
-
-
-
-
6
3
2
1
-
-
-
2
-
-
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
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-
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-
-
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6
-
3
2
1
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1
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649791
Kasuya
Inhibitory effect of quinolone ...
Mus musculus
Biochem. Pharmacol.
62
363-367
2001
-
-
-
-
-
-
8
-
2
-
-
1
-
2
-
-
1
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2
-
-
2
-
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-
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5
-
1
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1
8
5
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2
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-
1
-
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-
1
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650334
Morsczeck
The macrophage-induced gene (m ...
Mycobacterium avium
Biochim. Biophys. Acta
1521
59-65
2001
-
-
1
-
-
-
1
5
-
-
-
1
-
3
-
-
1
-
-
-
-
-
14
-
-
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-
-
-
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-
-
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1
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-
1
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5
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1
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-
1
-
-
-
-
14
-
-
-
-
-
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-
-
-
-
-
-
-
650950
Vessey
Isolation and preliminary char ...
Sus scrofa
Dig. Dis. Sci.
46
438-442
2001
-
-
-
-
-
-
1
2
-
2
1
-
-
1
-
-
-
-
-
1
-
-
8
-
-
-
-
-
2
-
-
-
1
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-
-
-
-
-
-
-
1
1
2
-
2
1
-
-
-
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-
1
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-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
650831
Kasuya
Characterization of a renal me ...
Mus musculus
Chem. Biol. Interact.
118
233-246
1999
-
-
-
-
-
-
3
-
2
-
-
1
-
2
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1
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2
1
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20
-
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3
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2
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1
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1
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2
1
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20
-
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-
-
-
-
-
649783
Kasuya
Molecular specificity of a med ...
Bos taurus
Biochem. Pharmacol.
55
1769-1775
1998
-
-
-
-
-
-
4
-
1
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-
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1
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1
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1
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20
-
-
-
-
-
-
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-
-
4
-
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-
-
-
-
-
-
-
4
4
-
1
-
-
-
-
-
-
1
-
1
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678
Kasuya
Purification and characterizat ...
Bos taurus
Drug Metab. Dispos.
24
879-883
1996
1
-
-
-
-
-
2
-
1
-
-
1
-
2
-
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1
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1
1
-
6
-
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1
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1
-
-
-
-
-
-
-
2
-
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1
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1
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1
-
1
1
-
6
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
679
Kasuya
Participation of a medium chai ...
Bos taurus
Biochem. Pharmacol.
51
805-809
1996
-
-
-
-
-
-
-
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1
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1
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2
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1
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1
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5
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1
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1
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1
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1
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5
-
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-
-
-
-
-
-
-
-
-
-
-
-
682
Kasuya
Inhibition of a medium chain a ...
Bos taurus
Biochem. Pharmacol.
52
1643-1646
1996
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
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1
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1
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-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
4
-
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-
-
-
-
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-
-
-
-
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681
Mao
Formation of a free acyl adeny ...
Rattus norvegicus
J. Biol. Chem.
267
3143-3146
1992
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1
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1
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2
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
668
Takao
Purification and kinetic prope ...
Byssochlamys spectabilis, Byssochlamys spectabilis AHU 9417
Agric. Biol. Chem.
51
145-152
1987
-
-
-
-
-
-
3
6
-
5
2
-
-
3
-
-
1
1
-
-
1
-
12
1
1
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1
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3
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6
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5
2
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1
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1
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12
1
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1
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1
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1
1
1
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1
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1
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1
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6
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2
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2
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1
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1
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1
1
1
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1
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1
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1
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1
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3
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1
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1
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3
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1
1
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4
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1
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1
1
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1
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562
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675
Scholte
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12
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12
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Bovine serum albumin activatio ...
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3
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1
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4
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1
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3
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1
3
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677
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1
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1
1
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8
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1
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2
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3
1
3
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1
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1
1
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8
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1
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